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Clean Sampling and Analysis of River and Estuarine Waters for Trace Metal Studies
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Flavobacterium amnigenum sp. nov. Isolated from a River
Kishor Sureshbhai Patil1, Shalem Raj Padakandla1,2, Jong-Chan Chae1
1Division of Biotechnology, Chonbuk National University, Iksan 54596, Republic of Korea.
Journal of Microbiology and Biotechnology
|August 17, 2018
Summary
A novel bacterium, *Flavobacterium amnigenum* sp. nov. strain I3-3T, was identified from Korean river water. This flexirubin-pigment-producing organism expands the known diversity within the *Flavobacterium* genus.
Area of Science:
- Microbiology
- Bacteriology
- Environmental microbiology
Background:
- River water ecosystems harbor diverse microbial communities.
- The genus *Flavobacterium* comprises Gram-negative bacteria with significant roles in various environments.
- Characterization of novel bacterial species is crucial for understanding microbial diversity and ecological functions.
Purpose of the Study:
- To isolate and characterize a novel bacterial strain from river water.
- To determine the taxonomic position of the novel isolate using polyphasic characterization.
- To propose a new species within the genus *Flavobacterium*.
Main Methods:
- Isolation and cultivation of bacteria from river water samples.
- Gram staining, motility tests, and assessment of catalase and oxidase activities.
- Determination of growth parameters (temperature, pH, NaCl tolerance).
- Analysis of major fatty acids, polar lipids, and respiratory quinones.
- Genomic DNA extraction and G+C content determination.
- 16S rRNA gene sequencing and phylogenetic analysis.
Main Results:
- A yellowish, flexirubin-pigment-producing, Gram-negative, aerobic, non-motile strain (I3-3T) was isolated.
- The strain exhibited catalase and oxidase activities and grew under specific ranges of temperature, pH, and salinity.
- Major fatty acids included iso-C15:0, iso-C17:0 3-OH, and summed feature 3.
- Polar lipids consisted of phosphatidylethanolamine and unidentified lipids; Menaquinone-6 (MK6) was the major respiratory quinone.
- The G+C content of the genomic DNA was 35.6%.
- Phylogenetic analysis based on 16S rRNA gene sequences showed highest similarity to *Flavobacterium nitrogenifigens* (96.95%) and *Flavobacterium compostarboris* (96.93%).
Conclusions:
- Strain I3-3T represents a distinct taxon within the genus *Flavobacterium* based on phenotypic and phylogenetic data.
- The proposed name for the novel species is *Flavobacterium amnigenum* sp. nov.
- The type strain is I3-3T (=KCTC 52884T =NBRC 112871T).
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