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Published on: March 30, 2018
[Effect of IKK2 Inhibitor LY2409881 on Diffuse Large B-Cell Lymphoma Cell Apoptosis and Its Mechanism]
Pu Yang1, Xiang Gao1, Jun Jiao1
1Institute of Blood Disease,Xuzhou Medical University,Xuzhou 221000,Jiangsu Province, China.Center of Diagnosis and Therapy for lymphoma, the Affiliated Hospital of Xuzhou Medical University,Xuzhou 221002,Jiangsu Province,China.
Objective:
To investigate the effects and its potential mechanism of IKK2 inhibitor LY2409881 on proliferation, cell cycle and apoptosis of diffuse large B-cell lymphoma (DLBCL) cell lines.
Methods:
CCK8 assay was used to detect the effect of LY2409881 on proliferation of DLBCL cell lines; Flow cytometry was used to analyze cell cycle; Western blot was used to analyze apoptosis and its potential mechanism.
Results:
LY2409881 inhibited the proliferation of multiple DLBCL cell lines obviously, and caused G1 cell arrest. Furtherly, LY2409881 inhibited the expression of c-FLIP, induced the activation of DR4 and caspase 8. Meanwhile, LY2409881 induced the expression of pro-apoptotic protein BAX and inhibited the expression of anti-apoptotic protein MCL-1 and BCL-2.
Conclusion:
LY2409881 inhibits the proliferation of DLBCL cell lines, causes G1 cell arrest and induces apoptosis via the endogenous and exogenous apoptotic pathways.
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