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Updated: Feb 6, 2026

Preparation and Gene Modification of Nonhuman Primate Hematopoietic Stem and Progenitor Cells
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[Advances of studies on Purification and Tracking of Hematopoietic Stem Cells Using Their Specific Gene

Min-Er Xie1, Fang Dong1, Tao Cheng2

  • 1State Key Laboratory of Experimental Hematology, Institute of Hematology& Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020,China.

Zhongguo Shi Yan Xue Ye Xue Za Zhi
|August 17, 2018
PubMed

Hematopoietic stem cells (HSC) maintain homeostatic hematopoiesis via their multi-lineage differentiation and self-renewal potentials. HSC can be enriched and purified by flow cytometry relying on their cell surface markers and functional characteristics, however, these methods can not meet the need for deep analysis of HSC biological property and function because of the poor purity. Recent studies have successfully purified and tracked HSC using specifically expressed genes, which can enhance the purification efficiency of HSC, thus provide a better tool for the in-vivo study of HSC. This review summarizes the new techniques and discusses their advantages and disadvantages.

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