Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

T cell-derived migration-inhibitory factor and colony-stimulating factor share common structural elements.

T Kawaguchi, D W Golde, A Mednis

    Blood
    |June 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Bcl-x<sub>L</sub> mediates RIPK3-dependent necrosis in M. tuberculosis-infected macrophages.

    Mucosal immunology·2017
    Same author

    Survival Experience of 195 Patients with Hairy Cell Leukemia Treated in a Multi-Institutional Study with Interferon-Alfa 2B.

    Leukemia & lymphoma·2016
    Same author

    Decrease of three lysosomal enzymes in guinea pig macrophages activated by lymphocyte mediators.

    Inflammation·2013
    Same author

    Apoptosis is an innate defense function of macrophages against Mycobacterium tuberculosis.

    Mucosal immunology·2011
    Same author

    Medical aid to honduras.

    Canadian Medical Association journal·2010
    Same author

    Dehydroascorbic acid, a blood-brain barrier transportable form of vitamin C, mediates potent cerebroprotection in experimental stroke.

    Proceedings of the National Academy of Sciences of the United States of America·2001
    Same journal

    Fibrocytes drive JAK2V617F-mutated myelofibrosis: pitavastatin reverses marrow fibrosis and anemia.

    Blood·2026
    Same journal

    Identifying steroid-refractory aGVHD before it happens.

    Blood·2026
    Same journal

    ELISA-negative HIT: antibody recognition and relevance.

    Blood·2026
    Same journal

    EBV and immunodeficiency: the odd couple drawn to the brain.

    Blood·2026
    Same journal

    A bone to pick with ferric carboxymaltose.

    Blood·2026
    Same journal

    A step toward streamlining HIT diagnosis.

    Blood·2026
    See all related articles

    Migration-inhibitory factor (MIF) and colony-stimulating factor (CSF) may be the same molecule. Purified MIF from Mo cells exhibited potent CSF activity, suggesting a shared identity or common structural elements.

    Area of Science:

    • Immunology
    • Molecular Biology
    • Hematopoiesis

    Background:

    • Migration-inhibitory factor (MIF) is a lymphokine involved in mononuclear phagocyte localization and activation.
    • Mo cells, a T cell leukemia virus II-infected line, secrete significant amounts of MIF upon stimulation.

    Purpose of the Study:

    • To purify Migration-inhibitory factor (MIF) from Mo cell-conditioned medium.
    • To investigate the functional activities of purified MIF.
    • To determine the relationship between MIF and colony-stimulating factor (CSF).

    Main Methods:

    • Purification of MIF using gel filtration, phenyl-Sepharose affinity chromatography, isoelectrofocusing, and RP-HPLC.
    • Assay of purified MIF for colony-stimulating factor (CSF) activity on human bone marrow cells.

    Related Experiment Videos

  • Characterization using C18-RP-HPLC and a monoclonal antibody against MIF.

    • Main Results:

    • MIF was purified with a 6,000-fold increase in specific activity.
    • The purified MIF fraction demonstrated potent CSF activity.
    • MIF and CSF activities showed coincident peaks during RP-HPLC and were both neutralized by an anti-MIF antibody.

    Conclusions:

    • Migration-inhibitory factor (MIF) and colony-stimulating factor (CSF) are likely identical or closely related molecules.
    • These findings suggest common structural elements between MIF and CSF.