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Phage vaccines displaying YGKDVKDLFDYAQE epitope induce protection against systemic candidiasis in mouse model

Hongxi Shi1, Shuai Dong2, Xintong Zhang2

  • 1Key Laboratory of Molecular Epigenetics of MOE and Institute of Genetics & Cytology, Northeast Normal University, Changchun, PR China.

Vaccine
|August 17, 2018
PubMed

Insights

Recombinant phages displaying a peptide from Fructose-bisphosphate aldolase 1 (Fba1) show promise as a novel vaccine. These engineered phages effectively protect against Candida albicans infections in mice, improving survival rates.

Area of Science:

  • Mycology
  • Immunology
  • Vaccine Development

Background:

  • Candida albicans is an opportunistic fungal pathogen causing significant health threats, particularly in immunocompromised individuals.
  • Current prophylactic and therapeutic strategies against candidiasis are limited.
  • A peptide from Fructose-bisphosphate aldolase 1 (Fba1) has shown potential as a vaccine against systemic candidiasis.

Purpose of the Study:

  • To display an epitope peptide (YGKDVKDLFDYAQE) from Fba1 on filamentous phage coat proteins (pIII or pVIII).
  • To investigate the protective effects of these recombinant phages against Candida albicans infections.
  • To evaluate the potential of phage-displayed peptides as a novel vaccine strategy.

Main Methods:

  • Epitope peptide YGKDVKDLFDYAQE was displayed on filamentous phage coat proteins (pIII and pVIII).
  • Mice were immunized with recombinant phages (phage-3F, phage-8F) or recombinant Fba1 (rFba1).
  • Immunized mice were challenged with Candida albicans yeast cells, and immune responses, fungal burden, kidney damage, and survival rates were assessed.

Main Results:

  • Recombinant phages and rFba1 successfully induced humoral and cellular immune responses in mice.
  • Immunization with recombinant phages significantly reduced fungal burden and kidney damage.
  • The developed vaccines markedly improved survival rates in mice challenged with Candida albicans.

Conclusions:

  • Recombinant phages displaying the Fba1-derived epitope YGKDVKDLFDYAQE are effective in protecting against Candida albicans infections.
  • These engineered phages represent a promising new vaccine candidate for candidiasis.
  • Phage display technology offers a viable platform for developing vaccines against fungal pathogens.
Keywords:
CandidiasisFba1Peptide vaccinePhage display

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