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Updated: Feb 6, 2026

Enhanced Oil Recovery using a Combination of Biosurfactants
Published on: June 3, 2022
Pullusurfactans A-E, new biosurfactants produced by Aureobasidium pullulans A11211-4-57 from a fleabane, Erigeron
Jong-Shik Kim1, In-Kyoung Lee2, Bong-Sik Yun3
1Gyeongbuk Institute for Marine Bio-industry, Uljin, Korea. jskim@gimb.or.kr.
Abstract:
Yeast biosurfactants have potent applications in medical, cosmeceutical, and food industries due to their specific modes of action, low toxicity, and applicability. In this study, biosurfactant-producing yeasts were screened for various industrial applications. Among them, Aureobasidium pullulans strain A11211-4-57 with potent surfactant activity from fleabane flower, Erigeron annus (L.) pers., was selected. From culture supernatant of strain A11211-4-57, five new low-surface-tension chemicals designated as pullusurfactans A-E were identified through consecutive chromatography steps, involving ODS, silica gel, Sephadex LH-20, and ODS Sep-pak cartridge columns. Based on mass and NMR measurements, structures of pullusurfactans A-E were determined as myo-inositol lipids with molecular formulae of C20H35O9, C18H32O8, C20H35O9, C24H42O9, and C18H32O8, respectively. These compounds exhibited potent biosurfactant activities (22.90, 22.40, 32.28, 25.28, and 22.44 mN/m, respectively). These results suggest that these novel biosurfactants have potential use as biosurfactants in industrial aspect.
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