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miR-202 suppresses prostate cancer growth and metastasis by targeting PIK3CA

Shengping Zhang1, Jiarong Cai2, Wenjun Xie3

  • 1Department of Urology, The People's Hospital of Longhua, Shenzhen, Guangdong 518109, P.R. China.

Experimental and Therapeutic Medicine
|August 17, 2018
PubMed

Insights

MicroRNA-202 (miR-202) acts as a tumor suppressor in prostate cancer. It is downregulated in tumors and inhibits cancer cell growth, migration, and invasion by targeting PIK3CA.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNA-202 (miR-202) is implicated in various human cancers.
  • Its role in prostate cancer pathogenesis is not well understood.

Purpose of the Study:

  • To investigate the function and mechanism of miR-202 in prostate cancer.

Main Methods:

  • Quantitative real-time PCR to assess miR-202 levels in prostate cancer tissues and cell lines.
  • In vitro assays (proliferation, migration, invasion, apoptosis) to evaluate miR-202's functional impact.
  • In vivo tumor xenograft models to assess miR-202's effect on tumor growth.
  • Western blotting and luciferase reporter assays to identify and validate miR-202 targets.

Main Results:

  • miR-202 expression was significantly downregulated in prostate cancer tissues and cell lines.
  • Overexpression of miR-202 inhibited prostate cancer cell proliferation, migration, and invasion, while promoting apoptosis.
  • miR-202 suppressed tumor growth in vivo.
  • Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit α (PIK3CA) was identified as a direct target of miR-202.
  • miR-202 overexpression reduced PIK3CA mRNA and protein levels.
  • Restoration of PIK3CA expression counteracted the inhibitory effects of miR-202 on prostate cancer cells.

Conclusions:

  • miR-202 functions as a tumor suppressor in prostate cancer.
  • miR-202 exerts its tumor-suppressive effects by directly targeting PIK3CA, thereby inhibiting cancer cell proliferation, migration, invasion, and promoting apoptosis.
Keywords:
PIK3CAmiR-202migrationproliferationprostate cancer

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