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Related Experiment Videos

Multiple sequence motifs are involved in SV40 enhancer function.

M Zenke, T Grundström, H Matthes

    The EMBO Journal
    |February 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

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    A study of the SV40 enhancer reveals it has two DNA domains that work together for significant transcription enhancement. Specific sequence motifs within these domains are key to their function and are found in other enhancers.

    Area of Science:

    • Molecular Biology
    • Genetics
    • Virology

    Background:

    • The Simian virus 40 (SV40) enhancer is a crucial regulatory DNA sequence.
    • Understanding enhancer function is vital for gene regulation studies.

    Purpose of the Study:

    • To systematically investigate the structure and function of the SV40 enhancer through mutagenesis.
    • To identify the key DNA domains and sequence motifs responsible for enhancer activity.

    Main Methods:

    • Systematic mutagenesis of the SV40 enhancer region.
    • Assessing transcriptional enhancement activity of modified enhancer sequences.
    • Analyzing sequence motifs within functional enhancer domains.

    Main Results:

    • The SV40 enhancer spans approximately 100 base pairs and comprises at least two distinct DNA domains.

    Related Experiment Videos

  • These domains exhibit minimal activity independently but cooperate for a 400-fold transcription enhancement.
  • Enhancer activity is largely independent of domain orientation and distance, and can be generated by domain duplication.
  • Specific sequence motifs within each domain are critical for enhancer function.

    • Conclusions:

    • The SV40 enhancer functions through the cooperative interaction of at least two distinct DNA domains.
    • Specific sequence motifs within these domains are essential and conserved across different enhancers.
    • This modular structure allows for flexibility in enhancer function and evolution.