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Updated: Feb 6, 2026

Quantitative Analysis of Aspergillus nidulans Growth Rate using Live Microscopy and Open-Source Software
Published on: July 24, 2021
Internuclear diffusion of histone H1 within cellular compartments of Aspergillus nidulans
Alexander P Mela1, Michelle Momany1
1Fungal Biology Group and Department of Plant Biology, University of Georgia, Athens, Georgia, United States of America.
Abstract:
Histone H1 is an evolutionarily conserved linker histone protein that functions in arranging and stabilizing chromatin structure and is frequently fused to a fluorescent protein to track nuclei in live cells. In time-lapse analyses, we observed stochastic exchange of photoactivated Dendra2-histone H1 protein between nuclei within the same cellular compartment. We also observed exchange of histones between genetically distinct nuclei in a heterokaryon derived from fusion of strains carrying histone H1-RFP or H1-GFP. Subsequent analysis of the resulting uninucleate conidia containing both RFP- and GFP-labeled histone H1 proteins showed only parental genotypes, ruling out genetic recombination and diploidization. These data together suggest that the linker histone H1 protein can diffuse between non-daughter nuclei in the filamentous fungus Aspergillus nidulans.
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