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Proteomic analysis of evodiamine-induced cytotoxicity in thyroid cancer cells

Hui-I Yu1, Hsiu-Chuan Chou2, Yi-Ching Su3

  • 1Division of Endocrinology and Metabolism, Department of Internal Medicine, Ditmanson Medical Foundation Chia-Yi Christian Hospital, Chiayi, Taiwan.

Journal of Pharmaceutical and Biomedical Analysis
|August 17, 2018
PubMed

Insights

Evodiamine shows potential for thyroid cancer treatment by impacting protein expression. This natural compound induces cytotoxicity through mechanisms involving protein folding, cytoskeleton, and transcription control in thyroid cancer cells.

Area of Science:

  • Natural Products Chemistry
  • Molecular Oncology
  • Proteomics

Background:

  • Evodiamine, a natural product from Tetradium, exhibits anti-fat uptake and anti-proliferation effects.
  • The impact of evodiamine on thyroid cancer remains largely unexplored.
  • Understanding evodiamine's mechanism is crucial for potential therapeutic applications.

Purpose of the Study:

  • To investigate the effects of evodiamine on human thyroid cancer cell line ARO.
  • To elucidate the cytotoxic mechanisms of evodiamine in thyroid cancer.
  • To identify potential therapeutic targets for thyroid cancer treatment.

Main Methods:

  • Lysine-labeling two-dimensional difference gel electrophoresis (2D-DIGE) was employed.
  • Mass spectrometry (MS) was used to analyze protein expression.
  • Differential protein expression was analyzed in evodiamine-treated ARO cells.

Main Results:

  • Evodiamine treatment significantly altered the expression of 77 protein features.
  • Cytotoxicity in thyroid cancer cells involved dysregulation of protein folding.
  • Evodiamine impacted cytoskeleton regulation and transcription control.

Conclusions:

  • Evodiamine induces cytotoxicity in thyroid cancer cells via multiple molecular pathways.
  • A combined proteomic strategy (2D-DIGE and MS) offers a rapid method for mechanism studies.
  • Identified protein targets warrant further investigation for thyroid cancer therapy.
Keywords:
2D-DIGEEvodiamineMALDI-TOFProteomicsThyroid cancer

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