A methodological approach to identify the most reliable human milk collection method for compositional analysis: a...

Area of Science:

• Human lactation research
• Nutritional science
• Infant health

Background:

• Breast milk composition varies significantly within and between women.
• Inconsistent sampling methodologies across studies complicate interpretation of breast milk macronutrient data.
• The impact of different breast milk collection techniques on reported composition is not well understood.

Purpose of the Study:

• To systematically review and compare breast milk macronutrient composition (protein, fat, lactose) across studies using diverse sampling methodologies.
• To identify the most robust and consistent breast milk sampling approach as an alternative to full expression.
• To establish a standardized protocol for human milk collection in research.

Main Methods:

• Comprehensive literature search of multiple databases (EMBASE, PubMed, Scopus, etc.).
• Inclusion of observational studies (cross-sectional, cohort) involving lactating women.
• Independent screening and quality assessment of studies using the Newcastle-Ottawa scale.

Main Results:

• Data not yet available as this is a protocol for a systematic review.
• The review will compare findings from studies employing different breast milk collection methods.
• Analysis will focus on macronutrient levels (protein, fat, lactose).

Conclusions:

• A reliable and practical human milk collection method is essential for accurate infant nutrition assessment.
• This systematic review aims to establish a standardized sampling protocol for future research.
• Standardization will enhance data sharing, replicability, and knowledge advancement in the field of breast milk composition.