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Related Concept Videos

Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
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Peptide Identification Using Tandem Mass Spectrometry01:33

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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Gas Chromatography–Mass Spectrometry (GC–MS)01:14

Gas Chromatography–Mass Spectrometry (GC–MS)

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Gas chromatography–mass spectrometry (GC–MS) is the combination of analytical techniques of gas chromatography and mass spectrometry in a single instrument for analyzing a mixture of compounds. The gas chromatograph separates the compounds in the mixture, and the mass spectrometer analyzes each compound separately to determine the molecular masses and molecular structures.
A gas chromatograph consists of a long, narrow capillary column with a polysiloxane coating on the inner wall....
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Special considerations while measuring pulse01:13

Special considerations while measuring pulse

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Assessing a patient's pulse is a fundamental skill in healthcare, but certain situations require special attention:
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Special considerations while measuring oxygen saturation01:19

Special considerations while measuring oxygen saturation

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Assessing respiratory rate concurrently with pulse measurement is fundamental to patient care, providing valuable insights into the patient's respiratory function. The normal breathing rate for an adult usually falls within a normal range of 12 to 20 breaths per minute. Abnormal respiratory rates can signal underlying health conditions or the need for immediate intervention.
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Special considerations while measuring blood pressure01:28

Special considerations while measuring blood pressure

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When assessing blood pressure (BP), healthcare professionals must consider various factors and potential unexpected outcomes to ensure accurate readings and provide proper patient care. Adhering to these guidelines is essential to achieving the most reliable results.
Monitoring Both Arms:
Monitoring BP in both arms during the initial assessment is advisable, as the systolic value may differ by five to ten mm Hg between arms. For subsequent BP assessments, use the arm with the higher reading.
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Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
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Special Considerations for Liquid Chromatography-Tandem Mass Spectrometry Method Development.

Brian A Rappold1

  • 1Mass Spectrometry Laboratory Corporation of America, 1904 TW Alexander Drive, Durham, NC 27703, USA.

Clinics in Laboratory Medicine
|August 18, 2018
PubMed
Summary

Developing diagnostic liquid-chromatography-tandem mass spectrometry (LC-MS/MS) assays requires careful consideration of intrinsic factors. Utilizing features like isotopically labeled internal standards and transition ratios builds confidence and speeds up validation for these crucial tests.

Keywords:
EndogenousInternal standardsLiquid chromatographyMass spectrometryMethod developmentValidation

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Clinical Diagnostics

Background:

  • Method development for diagnostic liquid-chromatography-tandem mass spectrometry (LC-MS/MS) assays is often learned through experience due to limited publication.
  • Key aspects of assay development beyond basic sample preparation, chromatography, and detection are not widely detailed.

Purpose of the Study:

  • To discuss critical aspects of LC-MS/MS assay development that are often overlooked.
  • To provide guidance on enhancing confidence and efficiency in diagnostic assay development.

Main Methods:

  • Focuses on intrinsic factors of LC-MS/MS technology during method development.
  • Highlights the use of isotopically labeled internal standards.
  • Emphasizes the appraisal of transition ratios for assay reliability.

Main Results:

  • Contemplating intrinsic LC-MS/MS factors improves assay development confidence.
  • Strategic use of internal standards and transition ratio analysis accelerates validation.
  • Experience-based learning in method development can be supplemented by structured guidance.

Conclusions:

  • Method development for diagnostic LC-MS/MS assays benefits from considering specific intrinsic factors.
  • Isotopically labeled internal standards and transition ratio analysis are vital for robust assay development.
  • This approach streamlines the path to assay validation and clinical testing.