Noble scallop, Chlamys nobilis, sperm motility duration in the post-activation phase

Sperm motility in the post-activation phase is important for conducting and assessing species-specific artificial fertilization protocols. This study characterizes spermatozoa movement of the noble scallop, Chlamys nobilis, during the post-activation phase. Sperm samples were diluted and activated by fresh seawater, and subsequently incubated at 26 °C for 4 h. Sperm movement variables including total motile sperm (TM), rapid sperm (RAP), curvilinear velocity (VCL), straight-line velocity (VSL), average path velocity (VAP), lateral head displacement (ALH) and beat-cross frequency (BCF) after sperm activation was recorded and analyzed using the computer assisted semen analyze system. Based on the motility index (MI), the sperm movement was categorized into four groupings (twitching before full activation, full activation, twitching after full activation, and decreasing during the latter portion of the sperm motility pattern). The full activation lasted 21 min with the greatest movement characteristics except BCF, and there was no difference with twitching before full activation except for the RAP. The greatest TM was observed at 24.5 min after activation. The RAP, VCL, VSL, VAP and ALH values in the post-activation phase increased at full activation, followed by a subsequent decrease, while the BCF continued to trend downward throughout the study. This study contributes to the understanding on the sperm property of the noble scallop for gamete management, fertilization and spat production in aquaculture.