Related Experiment Video

Updated: Feb 6, 2026

Ultrasound-Guided Induced Pluripotent Stem Cell-Derived Cardiomyocyte Implantation in Myocardial Infarcted Mice
08:03

Ultrasound-Guided Induced Pluripotent Stem Cell-Derived Cardiomyocyte Implantation in Myocardial Infarcted Mice

Published on: March 30, 2022

2.1K

Fluvastatin inhibits cardiomyocyte apoptosis after myocardial infarction through Toll pathway

Lili Jia1,2, Liang Wang3, Wenxue Liu4

  • 1Qilu Hospital of Shandong University, Jinan, Shandong 250012, P.R. China.

Experimental and Therapeutic Medicine
|August 18, 2018
PubMed

Insights

Fluzastatin treatment significantly reduced cardiomyocyte apoptosis following myocardial infarction in rats. This effect was associated with changes in Toll-like receptor 4 (TLR4) expression, suggesting a protective role.

Area of Science:

  • Cardiology
  • Pharmacology
  • Molecular Biology

Background:

  • Myocardial infarction (MI) leads to cardiomyocyte apoptosis.
  • Statins, like fluvastatin, are used to manage cardiovascular disease.
  • The role of Toll-like receptor 4 (TLR4) in MI-induced apoptosis requires further investigation.

Purpose of the Study:

  • To investigate the effect of fluvastatin on cardiomyocyte apoptosis in a rat model of myocardial infarction.
  • To explore the potential mechanism involving Toll-like receptor 4 (TLR4) expression.

Main Methods:

  • Establishment of rat models of myocardial infarction.
  • Treatment with fluvastatin (20 mg/kg/day) for one week.
  • Assessment of TLR4 mRNA and protein expression using RT-qPCR and Western blot.
  • Detection of cardiomyocyte apoptosis via TUNEL assay.

Main Results:

  • Fluvastatin treatment significantly reduced cardiomyocyte apoptosis (19.2±3.8%) compared to the MI group (P<0.05).
  • RT-qPCR showed significantly increased TLR4 mRNA expression in the fluvastatin and normal control groups compared to the MI group (P<0.05).
  • Western blot analysis revealed significantly decreased TLR4 protein expression in normal control, sham operation, and fluvastatin treatment groups compared to the MI group (P<0.05).

Conclusions:

  • Fluvastatin demonstrates a cardioprotective effect by inhibiting cardiomyocyte apoptosis after myocardial infarction in rats.
  • The mechanism may involve the modulation of Toll-like receptor 4 (TLR4) signaling pathways.
Keywords:
TLR4cardiomyocytefluvastatinmyocardial infarctionrat

More Related Videos

Acute Myocardial Infarction in Rats07:45

Acute Myocardial Infarction in Rats

Published on: February 16, 2011

54.7K
Induction of Myocardial Infarction in Adult Zebrafish Using Cryoinjury11:12

Induction of Myocardial Infarction in Adult Zebrafish Using Cryoinjury

Published on: April 18, 2012

23.0K

Related Concept Videos

Feedback Inhibition00:46

Feedback Inhibition

Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!
57.2K
Apoptosis01:30

Apoptosis

Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size...
15.2K
C4 Pathway and CAM01:27

C4 Pathway and CAM

Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
49.2K
Enzyme Inhibition01:30

Enzyme Inhibition

Inhibitors are molecules that reduce enzyme activity by binding to the enzyme. In a normally functioning cell, enzymes are regulated by a variety of inhibitors. Drugs and other toxins can also inhibit enzymes. Some inhibitors bind to the enzyme’s active site, while others inhibit enzymatic activity by binding to other sites on the protein structure.
92.6K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
6.0K
Other Glycolytic Pathways01:24

Other Glycolytic Pathways

The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...
922