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Superoxide anion generation during airway anaphylaxis
International Archives of Allergy and Applied Immunology
|January 1, 1986
Summary
Superoxide anion (O-2) release during anaphylaxis in guinea pig trachealis is linked to SRS-A activation. This generation is time- and concentration-dependent, and inhibited by SRS-A antagonists.
Area of Science:
- Immunology
- Pharmacology
- Respiratory Medicine
Background:
- Anaphylaxis involves complex mediator release.
- Superoxide anion (O-2) generation is implicated in inflammatory responses.
- The role of O-2 in airway anaphylaxis requires further elucidation.
Purpose of the Study:
- To investigate the generation of extracellular superoxide anion (O-2) during in vitro anaphylaxis in guinea pig trachealis.
- To determine the involvement of specific mediators and antagonists in O-2 release.
Main Methods:
- In vitro anaphylaxis model using guinea pig trachealis.
- Measurement of extracellular superoxide anion (O-2) generation.
- Treatment with diphenhydramine HCl, indomethacin, and FPL 55712.
Main Results:
- O-2 generation was time- and concentration-dependent.
- Diphenhydramine HCl did not affect O-2 release.
- Indomethacin augmented anaphylaxis tension but not O-2 release.
- FPL 55712 significantly inhibited O-2 generation.
Conclusions:
- Extracellular O-2 generation during airway anaphylaxis is primarily mediated by SRS-A products.
- SRS-A activation is a key pathway for O-2 release in this model.
- Targeting SRS-A may be a therapeutic strategy for anaphylaxis-induced airway inflammation.