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Integration Process for Protein Extraction from Microalgae Using Liquid Biphasic Electric Flotation (LBEF) System.

Revathy Sankaran1,2, Pau Loke Show3, Yu-Shen Cheng4

  • 1Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, 43500, Semenyih, Selangor Darul Ehsan, Malaysia. revathysankaran@ymail.com.

Molecular Biotechnology
|August 18, 2018
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Summary
This summary is machine-generated.

This study introduces a new, single-step method combining electrolysis and liquid biphasic flotation for efficient microalgae protein extraction. This integrated approach simplifies processing, reduces costs, and improves protein recovery from biomass.

Keywords:
ElectricIntegrationLiquid biphasic flotationMicroalgaeProtein

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Area of Science:

  • Biotechnology
  • Bioprocessing Engineering
  • Sustainable Resources

Background:

  • Microalgae are a valuable source of high-quality protein.
  • Conventional protein extraction methods are multi-step, complex, and costly.
  • Integrated cell disruption and protein recovery processes are of significant interest.

Purpose of the Study:

  • To investigate an integrated electrolysis and liquid biphasic flotation (LBEF) method for protein extraction from wet microalgae biomass.
  • To determine optimal operating conditions for efficient protein recovery from Chlorella sorokiniana CY-1.
  • To compare the efficacy of LBEF with and without electrolysis.

Main Methods:

  • Developed an integrated system combining electrolysis with liquid biphasic flotation (LBEF).
  • Optimized parameters including solvent composition (1-propanol, dipotassium hydrogen phosphate), microalgae loading, airflow, flotation time, and voltage.
  • Performed comparative analysis of LBEF with and without electrical assistance.

Main Results:

  • Achieved optimal protein recovery of 23.41% ± 1.25% and separation efficiency of 173.09% ± 4.48% under optimized conditions.
  • Demonstrated that electrolysis significantly enhances protein recovery and separation efficiency compared to LBEF alone.
  • Identified optimal conditions: 60% 1-propanol, 250 g/L K2HPO4, 0.1 g biomass, 150 cc/min airflow, 10 min, 20 V.

Conclusions:

  • The integrated electrolysis and LBEF method offers a simplified, cost-effective, and time-efficient approach for microalgae protein extraction.
  • This novel technique holds significant promise for industrial bioprocessing applications.
  • Electrolysis integration enhances the efficiency of microalgae protein recovery.