Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

13.7K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
13.7K
X-ray Crystallography02:18

X-ray Crystallography

26.2K
The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...
26.2K
Interference and Diffraction02:18

Interference and Diffraction

52.5K
Interference is a characteristic phenomenon exhibited by waves. When two electromagnetic waves interact with their peaks and troughs coinciding, a resulting wave with enhanced amplitude is produced. This is known as constructive interference. In this case, the two waves interacting are in phase with each other.
52.5K
RNA Interference01:23

RNA Interference

28.1K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
28.1K
Toughness and Hardness of Aggregate01:22

Toughness and Hardness of Aggregate

626
Toughness and hardness are critical properties of aggregate materials used in concrete, particularly on pavement surfaces and industrial flooring subjected to heavy loads. Toughness is defined as the aggregate's resistance to failure by impact and is measured by the aggregate impact value (AIV). For this, the aggregate impact value test is performed, wherein the impact is delivered by a standard hammer, which falls freely under its own weight onto the aggregates. The aggregates fragment in...
626
Interference and Decay01:16

Interference and Decay

477
Forgetting is a complex cognitive phenomenon influenced by several factors, among which interference and decay are particularly prominent. These processes explain why individuals often struggle to retrieve specific information from memory, leading to lapses in recall that can be observed in everyday situations.
Interference occurs when competing memories hinder the retrieval of particular information. It can be classified into two types: proactive and retroactive interference. Proactive...
477

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cloning and characterization of a transcription factor that binds to the proximal promoters of the two mouse type I collagen genes.

The Journal of biological chemistry·1997
Same author

Cerebrospinal fluid interleukin-6 in progressive Neuro-Behçet's syndrome.

Clinical immunology and immunopathology·1997
Same author

Experimental acute glomerulonephritis induced in the rabbit with a specific streptococcal antigen.

Clinical and experimental immunology·1997
Same author

Asymptomatic acute poststreptococcal glomerulonephritis following upper respiratory tract infections caused by Group A streptococci.

Clinical nephrology·1996
Same author

Fibrolipoma of the colon with an unusual appearance.

Endoscopy·1996
Same author

Histopathological changes in digits of dairy cows affected with sole ulcers.

The Journal of veterinary medical science·1996
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: Feb 6, 2026

Phase Contrast and Differential Interference Contrast DIC Microscopy
06:49

Phase Contrast and Differential Interference Contrast DIC Microscopy

Published on: August 6, 2008

54.1K

Differential interference contrast microscopy for cells using hard x-ray holography.

K Matsuda, J C A Lopez, S Rehman

    Applied Optics
    |August 18, 2018
    PubMed
    Summary
    This summary is machine-generated.

    We developed a new hard x-ray Gabor holography method for cell imaging. This technique uses spatial frequency filtering to create high-quality phase-contrast images without beam shearing.

    More Related Videos

    Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy
    08:54

    Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy

    Published on: June 5, 2019

    8.0K
    Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation
    07:54

    Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation

    Published on: March 12, 2015

    9.9K

    Related Experiment Videos

    Last Updated: Feb 6, 2026

    Phase Contrast and Differential Interference Contrast DIC Microscopy
    06:49

    Phase Contrast and Differential Interference Contrast DIC Microscopy

    Published on: August 6, 2008

    54.1K
    Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy
    08:54

    Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy

    Published on: June 5, 2019

    8.0K
    Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation
    07:54

    Preparing Adherent Cells for X-ray Fluorescence Imaging by Chemical Fixation

    Published on: March 12, 2015

    9.9K

    Area of Science:

    • Optics and Photonics
    • Biomedical Imaging
    • X-ray Microscopy

    Background:

    • Phase contrast microscopy enhances visibility of unstained, transparent specimens.
    • Traditional differential interference contrast (DIC) microscopy often requires beam shearing, which can be complex.
    • Hard X-ray microscopy offers high resolution for biological samples.

    Purpose of the Study:

    • To develop a novel differential interference contrast (DIC) imaging method for cells using hard x-ray Gabor holography.
    • To achieve phase contrast imaging without the need for beam shearing.
    • To demonstrate the capability of the method for imaging biological specimens.

    Main Methods:

    • Utilized hard x-ray Gabor holography to record phase information of a specimen.
    • Applied knife-edge filtering in the spatial frequency domain for image reconstruction.
    • Reconstructed phase-contrast images by manipulating holographic wavefronts in positive and negative frequency regions.

    Main Results:

    • Successfully generated phase-contrast images of 8-μm polystyrene beads.
    • Obtained good quality phase-contrast images of human HeLa cells.
    • Demonstrated effective interference and image formation without beam shearing.

    Conclusions:

    • The proposed method enables label-free, high-contrast imaging of cells using hard x-ray Gabor holography.
    • Spatial frequency domain filtering provides an alternative to beam shearing for DIC imaging.
    • This technique holds potential for advanced cellular imaging applications.