Related Experiment Video
Updated: Feb 6, 2026
Detection of Helicobacter pylori Infection and Antibiotic Resistance via Stool Quantitative Polymerase Chain Reaction Analysis
Published on: May 16, 2025
Effect of sequential therapy on treatment of Helicobacter pylori infection in children
Seyed Mohsen Dehghani1, Afsaneh Nazari2, Hazhir Javaherizadeh3
1Gastroenterohepatology Research Center, Nemazee Teaching Hospital, Shiraz University of Medical Sciences. Shiraz, Iran.
Insights
Sequential therapy effectively treated Helicobacter pylori infection in children, achieving an 82.5% eradication rate. This approach aids in healing gastritis and preventing ulcers by clearing the common H. pylori infection.
Area of Science:
- Pediatric Gastroenterology
- Infectious Diseases
- Microbiology
Background:
- Helicobacter pylori infection is a leading cause of chronic gastritis and duodenal ulcers globally.
- Eradicating H. pylori promotes gastric inflammation healing and peptic ulcer prevention.
- This infection is highly prevalent in both pediatric and adult populations.
Purpose of the Study:
- To assess the effectiveness of sequential therapy in treating Helicobacter pylori infections in children.
- To determine the eradication rate of a specific sequential treatment regimen.
Main Methods:
- A study involving 40 children diagnosed with H. pylori via endoscopy, biopsy, and rapid urease testing.
- The treatment involved a 10-day sequential therapy: Lansoprazole and Amoxicillin for 5 days, followed by Lansoprazole, Metronidazole, and Clarithromycin for 5 days.
- Eradication was confirmed using a stool antigen test six weeks post-treatment.
Main Results:
- The study included 40 children with a mean age of 10.8 years.
- Epigastric pain was the most frequent symptom (82.5%), with a mean duration of 16 months.
- Chronic gastritis was the most common pathological finding (77.5%), and the overall eradication rate was 82.5%. Nausea was the most frequent adverse effect (22.5%).
Conclusions:
- Sequential therapy demonstrated an 82.5% eradication rate for H. pylori in the studied pediatric cohort.
- This treatment regimen is effective in clearing H. pylori, contributing to the management of associated gastrointestinal conditions.
Background:
Helicobacter pylori infection which plays a major role in the etiology of chronic gastritis and duodenal ulcers in children and adults is one of the commonest chronic infection worldwide. Cure of the infection leads to healing of gastric inflammation and prevention of peptic ulcer.
Objective:
The aim of this study was to evaluate the efficacy of the sequential therapy for treatment of Helicobacter pylori infection.
Materials And Methods:
In this study, 40 children with symptoms of H. Pylori that the infection was proved by endoscopy and biopsy and rapid urease test (UBT) were enrolled, and received sequential therapy (Lansoprazol, Amoxicillin) for 5 days and (Lansoprazol, Metronidazole and Clarithromycin) for next 5 days. The eradication rate of therapy was evaluated by stool antigen test 6 weeks after completion of therapy. This study was carried out in Pediatric Gastroenterology Clinic of Shiraz University of Medical Sciences, Shiraz, Iran. This study was approved by ethic committee of Shiraz University of Medical Sciences.
Results:
Forty children with mean age of (10.8±4 years) were evaluated. The most common symptom on first admission was epigastric pain (82.5%), with mean duration of symptoms (16±14.5 month). The most common endoscopic findings was redness and erosion of the antrum (55%) and the most pathologic findings was chronic gastritis (77.5%). The most drug adverse effect was nausea (22.5%). The eradication rate of sequential therapy was 82.5%.
Conclusion:
Eradication rate of sequential therapy was 82.5% among our cases.
Related Concept Videos
01:16Treating Helicobacter pylori in Peptic Ulcers: Antimicrobial Therapy
00:59Gene Therapy
01:16Treatment for Pulmonary Arterial Hypertension: Oxygen Therapy for Respiratory Failure
Oxygen therapy is vital in increasing and maintaining blood oxygen levels in PAH patients. As a result, it aids in reducing fatigue,...
01:26Group Therapy
01:29Drug Dosing: Infants and Children
02:49Cancer Therapies
However, cancer treatments can pose several challenges, as therapies used to kill cancer cells are generally also toxic to normal cells. Moreover, cancer cells mutate rapidly and can develop resistance to chemical agents or radiation therapy. Besides, all types of cancer cells may not respond to the same therapy. Some cancer cells respond to one...