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Cation-dependent mannose-6-phosphate receptor functions as a pattern recognition receptor in anti-bacterial immunity

Ke-Yi Zhang1, Wen-Jie Yuan1, Ji-Dong Xu1

  • 1Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Qingdao, 266237, Shandong, China.

Developmental and Comparative Immunology
|August 18, 2018
PubMed

Insights

The cation-dependent mannose-6-phosphate receptor (CD-MPR) in shrimp acts as a pattern recognition receptor. It enhances antibacterial defense by triggering antimicrobial peptide expression against bacterial infections.

Area of Science:

  • Marine biology
  • Immunology
  • Biochemistry

Background:

  • The cation-dependent mannose-6-phosphate receptor (CD-MPR) is known for its role in lysosomal enzyme transport.
  • Its functions in immune responses, particularly in invertebrates, are not well understood.
  • This study investigates a CD-MPR-like molecule in the shrimp Marsupenaeus japonicus.

Purpose of the Study:

  • To identify and characterize a CD-MPR-like molecule in Marsupenaeus japonicus (MjCD-MPR).
  • To investigate the role of MjCD-MPR in the shrimp immune response against bacterial infection.
  • To elucidate the mechanism by which MjCD-MPR contributes to antibacterial defense.

Main Methods:

  • Identification and characterization of MjCD-MPR.
  • Gene expression analysis (mRNA and protein) after Vibrio anguillarum challenge.
  • RNA interference (RNAi) to knock down MjCD-MPR expression.
  • Binding assays with bacterial components (peptidoglycan, LPS, LTA).
  • Antimicrobial peptide (AMP) expression analysis.

Main Results:

  • MjCD-MPR expression was significantly upregulated upon Vibrio anguillarum challenge.
  • Knockdown of MjCD-MPR increased bacterial load, indicating a role in antibacterial defense.
  • MjCD-MPR binds to bacterial components like peptidoglycan and lipopolysaccharide.
  • MjCD-MPR knockdown led to decreased expression of key antimicrobial peptides (AMPs).

Conclusions:

  • MjCD-MPR functions as a pattern recognition receptor in shrimp innate immunity.
  • It plays a crucial role in recognizing and defending against bacterial infections.
  • MjCD-MPR promotes the expression of antimicrobial peptides, enhancing shrimp's defense mechanisms.
Keywords:
Antimicrobial peptideBacteriumCation-dependent mannose-6-phosphate receptorPolysaccharides

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