Linear relationship between cytoplasm resistance and hemoglobin in red blood cell hemolysis by electrical impedance
A Kiet Tran1, Achyut Sapkota2, Jianming Wen3
1Graduate School of Mechanical Engineering, Division of Artificial System Science, Chiba University, 1-33 Yayoi, Inage, Chiba 263-8522 Japan.
Abstract:
We have developed a non-invasive rapid and real-time red blood cell (RBC) hemolysis detection method which is a more accurate for point of care testing of hemolysis in various medical settings. An eight-parameter equivalent circuit is employed to quantify the release of hemoglobin (Hb) and the cytoplasm from RBC into the blood plasma. RBC hemolysis is induced by adding different volume fractions of distilled water into the blood. The cytoplasm released following RBC hemolysis is estimated from the experimental values. A strong relationship between RBC hemolysis and change in the electrical characteristics of blood has been demonstrated. The cytoplasm resistance (Rc) shows a linear relationship with the Hb. This relationship between Rc and Hb is described by the equation Rc = 0.2203Hb + 2.4775, with a correlation coefficient of 0.9905.
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