Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Five new insulin-producing cell lines with differing secretory properties.

C A Carrington, E D Rubery, E C Pearson

    The Journal of Endocrinology
    |May 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Type 2 (non-insulin-dependent) diabetes mellitus: the thrifty phenotype hypothesis. 1992.

    International journal of epidemiology·2013
    Same author

    The effect of maternal body condition score before and during pregnancy on the glucose tolerance of adult sheep offspring.

    Reproductive sciences (Thousand Oaks, Calif.)·2008
    Same author

    Effect of chronic MK-801 and/or phenytoin on the acquisition of complex behaviors in rats.

    Neurotoxicology and teratology·2007
    Same author

    Behavioral effects associated with chronic ketamine or remacemide exposure in rats.

    Neurotoxicology and teratology·2007
    Same author

    Hyperproinsulinaemia in normoglycaemic lipodystrophic HIV-infected patients.

    European journal of clinical investigation·2006
    Same author

    [From the economic genotype to the economic phenotype].

    Journees annuelles de diabetologie de l'Hotel-Dieu·2005

    Researchers developed five rat islet tumor cell lines with varying insulin and glucagon levels. These cell lines showed increased insulin and glucagon release in response to secretagogues and potassium, but not glucose.

    Area of Science:

    • Endocrinology
    • Cell Biology
    • Oncology

    Background:

    • Islet cell tumors are neoplasms of the pancreas.
    • Understanding the function of islet cells is crucial for diabetes research.
    • Tumor-derived cell lines provide valuable models for studying islet cell biology.

    Purpose of the Study:

    • To establish and characterize rat islet tumor-derived cell lines.
    • To investigate the insulin and glucagon secretory responses of these cell lines.

    Main Methods:

    • Derivation of five cell lines from a rat transplantable islet cell tumor.
    • Characterization of cell morphology, insulin, and glucagon content and release.
    • Stimulation of insulin and glucagon release using leucine, theophylline, potassium, and glucose.

    Related Experiment Videos

    Main Results:

    • Cell lines exhibited diverse morphology, insulin, and glucagon content/release profiles.
    • Leucine and theophylline significantly increased insulin release (approx. twofold).
    • High potassium induced a calcium-dependent increase in insulin and glucagon release (3-10 fold).
    • Glucose stimulation (2.8-16.7 mmol/l) did not affect insulin release.

    Conclusions:

    • Established rat islet tumor cell lines offer a model for studying islet cell secretion.
    • Secretagogue- and potassium-stimulated release mechanisms are functional in these cell lines.
    • The lack of glucose-stimulated insulin secretion suggests potential defects in glucose sensing pathways.