Application of deoxyribonucleic acid (DNA) polymorphisms to the analysis of DNA recovered from sperm

Sperms, collected following sexual activity of volunteers, were processed to isolate high-molecular weight deoxyribonucleic acid (DNA). These DNA samples were digested with particular restriction endonucleases and analyzed with probes that recognize polymorphic DNA regions within the human genome. The pattern of restriction fragment length polymorphisms (RFLP) detected by this test is identical to that observed with DNA prepared from blood of the male sexual partner. Therefore, RFLP analysis can be used to exclude or to determine the probable identity of an assailant in rape cases.