Novel quartz crystal microbalance immunodetection of aflatoxin B₁ coupling cargo-encapsulated liposome with...

A simple and sensitive quartz crystal microbalance (QCM) immunosensing platform was designed for the high-efficient detection of aflatoxin B₁ (AFB₁) in foodstuff. Initially, phenoxy-derived dextran molecule was immobilized on the surface of QCM gold substrate by using thiolated β-cyclodextrin based on the supramolecular host-guest chemistry between phenoxy group and cyclodextrin. Then, AFB₁-bovine serum albumin (AFB₁-BSA)-conjugated concanavalin A (Con A) was assembled onto the QCM probe through the dextran-Con A interaction. Glucose-loaded nanoliposome, labeled with monocolonal anti-AFB₁ antibody, was used for the amplification of QCM signal. Upon target AFB₁ introduction, the analyte competed with the immobilized AFB₁-BSA on the probe for the labeled anti-AFB₁ antibody on the nanoliposome. Based on specific antigen-antibody reaction, the amount of the conjugated nanoliposomes on the QCM probe gradually decreased with the increment of target AFB₁ in the sample. Upon injection of Triton X-100 in the detection cell, the carried nanoliposome was lysed to release the encapsulated glucose molecules. Thanks to the stronger affinity of Con A toward glucose than that of dextran, AFB₁-BSA-labeled Con A was displaced from the QCM probe, resulting in the change of the local frequency. Under the optimum conditions, the shift of the functionalized QCM immunosensing interface in the frequency shift was proportional to the concentration of target AFB₁ within a dynamic range from 1.0 ng kg^-1 to 10 μg kg^-1 at a low detection limit of 0.83 ng kg^-1. In addition, the acceptable assayed results on precision, reproducibility, specificity and method accuracy for the analysis of real samples were also acquired. Importantly, our strategy can provide a signal-on competitive immunoassay for the detection of small molecules, e.g., mycotoxins and biotoxins, thereby representing a versatile sensing schemes by controlling the corresponding antibody or hapten in the analysis of food safety.