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Virus-induced Gene Silencing VIGS in Nicotiana benthamiana and Tomato

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Published on: June 10, 2009

Antagonistic function of the Ve R-genes in tomato.

Ross N Nazar¹, Xin Xu², Alexander Kurosky³

¹Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, N1G 2W1, Canada. rnnazar@UoGuelph.ca.

Plant Molecular Biology

|August 20, 2018

Summary

Tomato Verticillium wilt resistance involves Ve1 and Ve2 proteins. Ve2 gene expression impacts defense proteins, but Ve1 protein negatively modulates these effects, suggesting an antagonistic relationship.

Keywords:

Gene silencing Plant defense Plant resistance Proteomics Receptor Vascular fungus Verticillium wilt

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Published on: March 11, 2020

Area of Science:

Plant Pathology
Molecular Biology
Genetics

Background:

Verticillium wilt is a major tomato disease.
Resistance is controlled by the Ve R-gene locus, encoding Ve1 and Ve2 receptor-like proteins.
Ve1 is induced during infection, while Ve2 is constitutive; Ve1 is typically credited with resistance.

Purpose of the Study:

To investigate the function of the Ve2 gene in tomato Verticillium wilt resistance.
To determine the relationship between Ve1 and Ve2 protein functions.

Main Methods:

RNA interference (RNAi) was used to suppress Ve2 mRNA levels in tomato near-isolines.
Changes in defense/stress protein levels were evaluated at both mRNA and protein levels.

Main Results:

Ve2 gene expression significantly affects numerous defense/stress protein levels.
The presence of functional Ve1 protein counteracts or minimizes the effects of Ve2 expression.
An antagonistic interaction between Ve1 and Ve2 proteins was observed.

Conclusions:

Ve2 plays a crucial role in modulating defense/stress protein responses.
Ve1 acts antagonistically to Ve2, negatively regulating the induction of defense/stress proteins.
The Ve R-gene locus functions through a complex interplay between Ve1 and Ve2 proteins.