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Updated: Feb 6, 2026

Isolation and Chemical Characterization of Lipid A from Gram-negative Bacteria
Published on: September 16, 2013
A latent green fluorescent styrylcoumarin probe for the selective growth and detection of Gram negative bacteria
Linda Váradi1, Miaoyi Wang1, Ramesh R Mamidi1
1Faculty of Medicine and Health, The University of Sydney School of Pharmacy, Camperdown Campus, NSW 2006, Australia.
Abstract:
A novel, green fluorescent β-alanylstyrylcoumarin derivative was synthesized and evaluated for its performance as a fluorogenic enzyme substrate on a range of clinically relevant microorganisms. The substrate was selectively hydrolysed by β-alanyl aminopeptidase producing P. aeruginosa resulting in an on-to-off fluorescent signal. Growth inhibitory effect of the substrate was observed on Gram positive bacteria and yeasts. Meanwhile, Gram negative species, despite their extremely protective cell envelope, showed ready uptake and accumulation of the substrate within their healthy growing colonies displaying intense green fluorescence.
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