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Published on: July 22, 2019
Novel Epigenetic Techniques Provided by the CRISPR/Cas9 System
Nina Xie1,2, Yafang Zhou1,2, Qiying Sun1,2
1Department of Geriatric Neurology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.
Abstract:
Epigenetics classically refers to the inheritable changes of hereditary information without perturbing DNA sequences. Understanding mechanisms of how epigenetic factors contribute to inheritable phenotype changes and cell identity will pave the way for us to understand diverse biological processes. In recent years, the emergence of CRISPR/Cas9 technology has provided us with new routes to the epigenetic field. In this review, novel epigenetic techniques utilizing the CRISPR/Cas9 system are the main contents to be discussed, including epigenome editing, temporal and spatial control of epigenetic effectors, noncoding RNA manipulation, chromatin in vivo imaging, and epigenetic element screening.
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