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Updated: Feb 6, 2026

Development of Metarhizium anisopliae as a Mycoinsecticide: From Isolation to Field Performance
Published on: July 30, 2017
Characterisation and evaluation of Metarhizium anisopliae (Metsch.) Sorokin strains for their temperature tolerance
K N P Chandra Teja1,2, S J Rahman3
1Department of Microbiology, Agri Biotech Foundation, Hyderabad, India.
Abstract:
Entomopathogenic fungal species from the genera Beauveria, Metarhizium and Lecanicillium are important components in biological control of insect pests. However, temperature, humidity and UV radiation are among the important abiotic factors, which limit their effective usage. In this study, four local isolates of Metarhizium were isolated from different crop rhiospheres of Telangana and Andhra Pradesh states of India, identified and tested for their temperature tolerance in terms of radial growth, conidial yield per 10 mm disc and rate of conidial germination at different incubation temperatures. The results revealed that strains LaMa1 and MaAICRP performed well in terms of radial growth, conidial yield and rate of conidial germination, even at 35°C temperature. The role of such temperature tolerant strains in agriculture is discussed.
Insights
This study identified temperature-tolerant Metarhizium fungal isolates for biological pest control. Strains LaMa1 and MaAICRP showed excellent performance at 35°C, offering potential for agricultural applications.
Area of Science:
- Agricultural Science
- Mycology
- Biological Control
Background:
- Entomopathogenic fungi, including Beauveria, Metarhizium, and Lecanicillium, are vital for insect pest biological control.
- Abiotic factors such as temperature, humidity, and UV radiation significantly limit the efficacy of these biocontrol agents.
Purpose of the Study:
- To isolate and identify local Metarhizium fungal strains from Indian crop rhizospheres.
- To evaluate the temperature tolerance of these Metarhizium isolates.
Main Methods:
- Isolation of four Metarhizium strains from Telangana and Andhra Pradesh crop rhizospheres.
- Assessment of radial growth, conidial yield, and germination rates at various incubation temperatures.
- Identification of promising temperature-tolerant strains.
Main Results:
- Two strains, LaMa1 and MaAICRP, demonstrated robust performance.
- These selected strains exhibited favorable radial growth, high conidial yield, and rapid germination even at 35°C.
- This indicates significant heat tolerance.
Conclusions:
- The identified Metarhizium strains (LaMa1 and MaAICRP) possess high temperature tolerance.
- These strains hold promise for enhanced biological control strategies in agriculture, particularly in warmer climates.
- Further research should explore their field efficacy and integration into pest management programs.
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