Related Experiment Video
Updated: Feb 6, 2026

A Method to Study the C924T Polymorphism of the Thromboxane A2 Receptor Gene
Published on: April 1, 2019
Polymorphisms in protamine 1 and 2 genes in asthenozoospermic men: A case-control study
Ali Nabi1, Mohammad Ali Khalili1,2, Mojgan Moshrefi1
1Research and Clinical Center for Infertility, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Genetic variations in protamine genes (PRM1 and PRM2) were investigated in men with asthenozoospermia. Some specific gene polymorphisms showed a slightly higher frequency in infertile men, suggesting a potential role in male infertility.
Area of Science:
- Reproductive biology and genetics.
- Molecular diagnostics and male infertility research.
Background:
- Asthenozoospermia, a cause of male infertility, may be linked to protamine gene abnormalities.
- Protamine deficiency or reduced protamine transcript levels are implicated in asthenozoospermia.
Purpose of the Study:
- To evaluate protamine-1 (PRM1) and protamine-2 (PRM2) gene polymorphisms in men diagnosed with asthenozoospermia.
- To identify potential genetic markers associated with asthenozoospermia.
Main Methods:
- A case-control study comparing asthenozoospermic men (cases) with normozoospermic men (controls).
- DNA sequencing of PRM1 and PRM2 genes using specific primers to screen for polymorphisms.
- Analysis of allele frequencies of identified single nucleotide polymorphisms (SNPs).
Main Results:
- Nine prevalent polymorphism regions in PRM1 and PRM2 genes were identified.
- Specific polymorphisms in PRM1 (139C>A) and PRM2 (373C>A, 298G>C) showed higher allele frequencies in asthenozoospermic individuals.
- The study identified several highly prevalent polymorphism regions within the PRM1 and PRM2 genes.
Conclusions:
- Certain altered genotypes in PRM1 and PRM2 genes were found at a slightly higher frequency in asthenozoospermic men compared to controls.
- Further extensive research is recommended to confirm these genotypes as reliable molecular markers for asthenozoospermia diagnosis.
More Related Videos
07:26High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment
Published on: July 18, 2017
07:24Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
Published on: February 10, 2023
Related Concept Videos
Combinatorial Gene Control
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
Predicting Products: SN1 vs. SN2
With increased substitution on the alkyl halide,...
Types of Biopharmaceutical Studies: Controlled and Non-Controlled Approaches
Non-controlled studies, commonly employed for initial exploration, lack a control group, rendering them susceptible to biases and external influences. In contrast,...
Single Nucleotide Polymorphisms-SNPs
1° Amines to Diazonium or Aryldiazonium Salts: Diazotization with NaNO2 Overview
The nitrous acid is unstable. Hence, it is formed in situ from a solution of sodium nitrite and cold aqueous acids such as hydrochloric or sulfuric acid. In an acidic solution, the –OH group of nitrous acid undergoes protonation to give oxonium ion, followed by...
1° Amines to Diazonium or Aryldiazonium Salts: Diazotization with NaNO2 Mechanism