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Related Experiment Video

Updated: Feb 6, 2026

Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion

Super-Resolution Imaging of Bacterial Secreted Proteins Using Genetic Code Expansion

Published on: February 10, 2023

Genetic Code Expansion in Animals.

Wes Brown¹, Jihe Liu¹, Alexander Deiters¹

¹Department of Chemistry , University of Pittsburgh , 219 Parkman Avenue , Pittsburgh , Pennsylvania 15237 , United States.

ACS Chemical Biology

|August 21, 2018

Summary

This summary is machine-generated.

Genetic code expansion allows incorporating unnatural amino acids into proteins in animals. This review covers recent advances, applications like proteomic labeling, and future directions for this powerful biological tool.

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Area of Science:

Biochemistry
Molecular Biology
Genetics

Background:

Genetic code expansion enables the synthesis of proteins with unnatural amino acids.
This technology, previously limited to microbial and mammalian cells, is now being applied in animal models.
Recent progress has extended its use to organisms like worms, fruit flies, zebrafish, and mice.

Purpose of the Study:

To review recent advancements in genetic code expansion methodology in animal models.
To highlight key applications of this technology in various animal systems.
To discuss current challenges and future prospects for unnatural amino acid mutagenesis in animals.

Main Methods:

Review of recent literature on genetic code expansion in animal models.
Analysis of specific applications such as proteomic labeling and optical protein control.
Summary of challenges and future research directions.

Main Results:

Significant progress has been made in adapting genetic code expansion for use in diverse animal models.
Applications include in vivo proteomic labeling in fruit flies and mice.
Optical control of protein function has been demonstrated in mice and zebrafish.

Conclusions:

Genetic code expansion is a rapidly advancing technology with broad potential in animal research.
Overcoming challenges in unnatural amino acid mutagenesis is key to wider adoption.
This technique offers powerful new avenues for studying protein structure and function in vivo.