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Updated: Feb 6, 2026
Transoral Robotic Total Thyroidectomy and Bilateral Central Regional Lymph Node Dissection for Papillary Thyroid Carcinoma
Published on: September 15, 2023
miR-497 inhibited proliferation, migration and invasion of thyroid papillary carcinoma cells by negatively regulating
Hui Cheng1, Hui Dong1, Jianlin Feng2
1Department of Pathology, The People's Hospital of Shouguang, Weifang 262700, China.
Purpose:
This article aimed to investigate the effect of miR-497 on thyroid papillary carcinoma.
Materials And Methods:
miR-497 expression was analyzed using The Cancer Genome Atlas. A total of 56 papillary thyroid carcinoma patients' tumor tissues and normal tissues were collected. Nthy-ori 3-1 and K1 cells were cultured. K1 cells were also transfected. Quantitative real-time polymerase chain reaction and Western blot were used to detect miR-497 and yes-associated protein 1 (YAP1) expression. Luciferase reporter assay was performed. MTT and Transwell assay were conducted to measure cells' proliferation, migration and invasion. Immunofluorescence detection was used to detect YAP1-positive cells.
Results:
miR-497 was downregulated, while YAP1 was upregulated in thyroid papillary carcinoma tissues and K1 cells (P<0.05). Compared with the negative control group, the OD495 value and the migrating and invasive cell number were significantly lower in miR-497 mimics group and significantly higher in miR-497 inhibitor group (P<0.05). YAP1 was the target gene of miR-497. Compared with blank group, the OD495 value and the migrating and invasive cell number were significantly lower in si-YAP1 group and significantly higher in miR-497 inhibitor group (P<0.05), while no significant difference was found between si-YAP1+inhibitors group and blank group in these indicators.
Conclusion:
miR-497 regulated the proliferation, migration and invasion of K1 cells by negatively regulating YAP1 expression.
Insights
MicroRNA-497 (miR-497) is downregulated in thyroid papillary carcinoma, suppressing tumor cell proliferation and migration. It targets yes-associated protein 1 (YAP1), indicating miR-497 as a potential therapeutic target.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Thyroid papillary carcinoma is a prevalent endocrine malignancy.
- Dysregulation of microRNAs (miRNAs) is implicated in cancer development.
- The role of miR-497 in thyroid papillary carcinoma remains to be fully elucidated.
Purpose of the Study:
- To investigate the expression and function of miR-497 in thyroid papillary carcinoma.
- To determine the relationship between miR-497 and yes-associated protein 1 (YAP1) in this cancer.
- To explore the impact of miR-497 on cancer cell behavior.
Main Methods:
- Analysis of The Cancer Genome Atlas data for miR-497 expression.
- Quantitative real-time polymerase chain reaction and Western blot to assess miR-497 and YAP1 levels.
- In vitro assays (MTT, Transwell) to evaluate cell proliferation, migration, and invasion.
- Luciferase reporter assay to confirm YAP1 as a direct target of miR-497.
Main Results:
- miR-497 was significantly downregulated in thyroid papillary carcinoma tissues and cells.
- Overexpression of miR-497 inhibited, while inhibition of miR-497 promoted, cell proliferation, migration, and invasion.
- YAP1 was identified as a direct target of miR-497, with inverse expression correlation.
- Silencing YAP1 mimicked the suppressive effects of miR-497, and miR-497 inhibition reversed these effects.
Conclusions:
- miR-497 plays a tumor-suppressive role in thyroid papillary carcinoma.
- miR-497 regulates cancer cell proliferation, migration, and invasion by targeting YAP1.
- miR-497 represents a potential therapeutic biomarker for thyroid papillary carcinoma.
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