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Multipronged Phenotyping Approaches to Characterize Sugarcane Root Systems
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A synthetic medium to simulate sugarcane molasses.

Felipe Senne de Oliveira Lino1, Thiago Olitta Basso2, Morten Otto Alexander Sommer1

  • 11Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitovert 220, 2800 Kongens Lyngby, Denmark.

Biotechnology for Biofuels
|August 22, 2018
PubMed
Summary

A new synthetic medium accurately simulates sugarcane molasses, enabling early-stage testing of microbial cell factories under industrial conditions. This development improves the efficiency of creating yeast strains for biofuel production.

Keywords:
Industrial strainsMicrobial physiologyPairwise cultivationStrain fitnessSynthetic molassesYeast fermentation

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Area of Science:

  • Biotechnology
  • Industrial Microbiology
  • Synthetic Biology

Background:

  • Developing microbial cell factories requires testing under industrial conditions, often done late in strain development.
  • Sugarcane molasses is a key substrate for bioprocesses, but no defined laboratory media accurately simulate it.
  • Early-stage testing in relevant conditions can accelerate the development of microbial cell factories.

Purpose of the Study:

  • To develop and validate a synthetic medium that accurately simulates industrial sugarcane molasses.
  • To assess the performance of this synthetic medium in laboratory-scale fermentation experiments.
  • To evaluate the impact of the synthetic medium on yeast strain fitness and fermentation parameters.

Main Methods:

  • Developed a synthetic medium (SM) to mimic sugarcane molasses composition.
  • Conducted laboratory-scale fermentations using industrial yeast strains (PE-2, Ethanol Red™) with both SM and actual molasses.
  • Performed sequential pairwise competition experiments to assess strain fitness in different media.

Main Results:

  • Fermentation performance (ethanol yield, biomass, viability, by-product formation) was reproducible between SM and molasses-based media.
  • Ethanol yields in SM ranged from 0.43–0.44 g/g, comparable to molasses (0.40–0.46 g/g).
  • Competition experiments showed SM significantly impacted yeast strain fitness, with a laboratory strain's abundance decreasing 5-fold compared to a standard lab medium.

Conclusions:

  • A novel synthetic medium effectively simulates industrial sugarcane molasses conditions.
  • The synthetic medium allows for reliable laboratory-scale testing of microbial cell factories.
  • This tool facilitates efficient development and selection of yeast strains for bioprocesses like biofuel production.