Related Experiment Video

Updated: Feb 6, 2026

Gene Expression Analyses in Human Follicles
09:09

Gene Expression Analyses in Human Follicles

Published on: February 17, 2023

1.5K

Repercussion of nonsteroidal anti-inflammatory drugs on the gene expression of human osteoblasts

Lucia Melguizo-Rodríguez1,2, Víctor J Costela-Ruiz1, Francisco J Manzano-Moreno2,3

  • 1Biomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences, University of Granada, Granada, Spain.

Peerj
|August 22, 2018
PubMed
Abstract

Insights

Nonsteroidal anti-inflammatory drugs (NSAIDs) negatively impact osteoblast gene expression, affecting bone health. This study analyzed how various NSAIDs influence key markers of osteoblast maturation and function.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Bone Biology

Background:

  • Nonsteroidal anti-inflammatory drugs (NSAIDs) are widely used and can adversely affect osteoblasts.
  • Understanding NSAID impact on bone cells is crucial for clinical practice.

Purpose of the Study:

  • To investigate the effects of various NSAIDs on osteoblast gene expression.
  • To analyze the impact of NSAIDs on markers of osteoblast maturation and function.

Main Methods:

  • Human osteoblast cell lines were treated with 10 µM concentrations of eight different NSAIDs.
  • Real-time PCR was used to analyze gene expression of key osteoblast markers, including RUNX-2, COL-I, OSX, OSC, BMP-2, BMP-7, and TGF-β signaling pathway components.

Main Results:

  • All tested NSAIDs reduced the expression of RUNX-2, COL-I, and OSX.
  • Most NSAIDs decreased OSC and BMP-7 expression, while BMP-2 was affected by all except naproxen.
  • NSAID treatment generally increased TGF-β1 expression but did not significantly alter its receptors.

Conclusions:

  • NSAIDs demonstrably affect osteoblast physiology.
  • These findings suggest a potential negative impact of NSAIDs on bone health and remodeling.
Keywords:
Bone tissueDifferentiationGene expressionNSAIDsOsteoblasts

More Related Videos

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells10:34

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells

Published on: April 14, 2010

16.0K
Drug Treatment and In Vivo Imaging of Osteoblast-Osteoclast Interactions in a Medaka Fish Osteoporosis Model08:53

Drug Treatment and In Vivo Imaging of Osteoblast-Osteoclast Interactions in a Medaka Fish Osteoporosis Model

Published on: January 1, 2017

9.8K

Related Concept Videos

What is Gene Expression?01:42

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
196.9K
What is Gene Expression?01:36

What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
11.5K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.6K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

5.6K
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
24.9K
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
6.7K