Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Adult Stem Cells01:33

Adult Stem Cells

33.9K
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
33.9K
Embryonic Stem Cells00:58

Embryonic Stem Cells

32.5K
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
32.5K
Embryonic Stem Cells00:57

Embryonic Stem Cells

5.1K
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
5.1K
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

28.1K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
28.1K
What is Genetic Engineering?00:49

What is Genetic Engineering?

80.3K
Overview
80.3K
Overview of Advanced Functional Groups02:22

Overview of Advanced Functional Groups

30.1K

Functional groups are groups of atoms with specific chemical properties that occur within organic molecules and are sometimes denoted as “R”. Functional groups can “functionalize” a compound by enabling it to adopt different physical and chemical properties.
Types of Advanced Functional Groups
The table below summarizes some of the major functional groups in organic chemistry.
30.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Local versus general anaesthesia for adults undergoing pars plana vitrectomy surgery.

The Cochrane database of systematic reviews·2016
Same author

Immunodominant SARS Coronavirus Epitopes in Humans Elicited both Enhancing and Neutralizing Effects on Infection in Non-human Primates.

ACS infectious diseases·2016
Same author

Effect of nonylphenol on volatile fatty acids accumulation during anaerobic fermentation of waste activated sludge.

Water research·2016
Same author

Delivery of siRNA Using Lipid Nanoparticles Modified with Cell Penetrating Peptide.

ACS applied materials & interfaces·2016
Same author

Steroidogenic Acute Regulatory Protein Overexpression Correlates with Protein Kinase A Activation in Adrenocortical Adenoma.

PloS one·2016
Same author

An Effective Molecular Target Site in Hepatitis B Virus S Gene for Cas9 Cleavage and Mutational Inactivation.

International journal of biological sciences·2016

Related Experiment Video

Updated: Feb 6, 2026

Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells iPSCs Using Retroviral Vector with GFP
08:25

Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells iPSCs Using Retroviral Vector with GFP

Published on: April 3, 2012

21.1K

Recent Advances in Engineering the Stem Cell Microniche in 3D.

Min Bao1, Jing Xie1, Wilhelm T S Huck1

  • 1Institute for Molecules and Materials Radboud University Heyendaalseweg 135 6525 AJ Nijmegen The Netherlands.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|August 22, 2018
PubMed
Summary

Bioinspired 3D matrices better mimic the in vivo cellular microenvironment than traditional 2D cultures. Engineering these 3D environments precisely controls properties, advancing our understanding of cell function.

Keywords:
3D cell culturescell geometriesdimensionalitymechanotransductionmicroenvironments

More Related Videos

Engineering Cell-permeable Protein
21:08

Engineering Cell-permeable Protein

Published on: December 28, 2009

15.0K
Alginate Microcapsule as a 3D Platform for Propagation and Differentiation of Human Embryonic Stem Cells hESC to Different Lineages
10:01

Alginate Microcapsule as a 3D Platform for Propagation and Differentiation of Human Embryonic Stem Cells hESC to Different Lineages

Published on: March 9, 2012

16.7K

Related Experiment Videos

Last Updated: Feb 6, 2026

Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells iPSCs Using Retroviral Vector with GFP
08:25

Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells iPSCs Using Retroviral Vector with GFP

Published on: April 3, 2012

21.1K
Engineering Cell-permeable Protein
21:08

Engineering Cell-permeable Protein

Published on: December 28, 2009

15.0K
Alginate Microcapsule as a 3D Platform for Propagation and Differentiation of Human Embryonic Stem Cells hESC to Different Lineages
10:01

Alginate Microcapsule as a 3D Platform for Propagation and Differentiation of Human Embryonic Stem Cells hESC to Different Lineages

Published on: March 9, 2012

16.7K

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Tissue Engineering

Background:

  • Conventional 2D cell culture provides insights into cellular mechanisms but fails to replicate the native in vivo microenvironment's physical and chemical properties.
  • Cells cultured in vitro on 2D substrates exhibit significantly different behaviors compared to those grown in vivo.
  • There is a critical need for advanced cell culture models that more accurately represent the natural cellular milieu.

Purpose of the Study:

  • To review recent advancements in bioinspired 3D matrices for recapitulating the natural extracellular matrix.
  • To discuss techniques for engineering 3D microenvironments with controlled biophysical and chemical properties.
  • To explore the impact of these engineered 3D environments on cellular behavior and function.

Main Methods:

  • Review of literature on bioinspired 3D matrices and their fabrication.
  • Analysis of techniques for controlling biophysical and chemical properties of 3D cell culture models.
  • Examination of studies investigating cellular responses within engineered 3D microenvironments.

Main Results:

  • 3D matrices can be engineered to closely mimic the native extracellular matrix, offering a more physiologically relevant in vitro model.
  • Precisely controlled biophysical and chemical cues within 3D environments significantly influence cell adhesion, spreading, proliferation, and differentiation.
  • These advanced models provide deeper insights into cell behavior compared to traditional 2D cultures.

Conclusions:

  • Bioinspired 3D matrices represent a significant improvement over 2D cultures for studying cell behavior in a microenvironment that closely resembles in vivo conditions.
  • Engineering sophisticated 3D microenvironments is crucial for understanding the complex interplay between cells and their surroundings.
  • Future research in 3D microenvironment engineering will further elucidate the influence of the microenvironment on cell function and disease pathology.