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Updated: Feb 6, 2026

Evaluation of the Storage Stability of Extracellular Vesicles
Published on: May 22, 2019
Changes occurring in stabilized ultra-high-temperature-treated whole milk during storage
Fernando César Dos Santos1, Fabiano Érisson da Cunha2, Érika de Pádua Alves1
1Dairy Science and Technology Graduate Programme, University North of Paraná (UNOPAR),Londrina, 86041-100,Brazil.
The study reveals a strong correlation between casein and free fatty acids (FFAs) in ultra-high temperature-treated (UHT) whole milk over its shelf-life. Casein degradation occurs at a rate of 0.021 g/100 g per day, with complete breakdown estimated by day 560.
Area of Science:
- Dairy Science
- Food Chemistry
- Microbiology
Background:
- Ultra-high temperature (UHT) treatment extends milk shelf-life but can impact milk components.
- Casein and free fatty acids (FFAs) are key components affecting UHT milk quality during storage.
- Understanding the degradation rates of these components is crucial for predicting shelf-life and quality.
Purpose of the Study:
- To investigate the relationship between casein, FFAs, and storage time in UHT whole milk.
- To estimate the daily rate of casein degradation in UHT whole milk.
- To determine the total time for complete casein degradation.
Main Methods:
- Samples of UHT whole milk were collected over a 120-day period.
- Raw milk quality was assessed via total mesophilic (TMC), psychrotrophic (TPC), and somatic cell counts (SCC).
- UHT milk composition was analyzed for fat, sialic acid, casein, and FFA content.
Main Results:
- A significant negative correlation was found between casein content and storage time (r = -0.991).
- A significant positive correlation was observed between FFA content and storage time (r = 0.962).
- The estimated daily rate of casein hydrolysis was 0.021 g/100 g, with complete degradation predicted by day 560.
Conclusions:
- Microbiological quality and SCC of raw milk are critical factors influencing casein and lipid integrity in UHT milk.
- The degradation of casein and the increase in FFAs are directly linked to the storage duration of UHT milk.
- These findings contribute to a better understanding of UHT milk stability and shelf-life prediction.
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