Soluble Mediators From Lactobacillus rhamnosus Gorbach-Goldin Support Intestinal Barrier Function in Rats After...

Area of Science:

• Gastroenterology
• Microbiology
• Immunology

Background:

• The intestinal barrier is crucial for gastrointestinal health.
• Short bowel syndrome (SBS) significantly compromises intestinal barrier function.
• Lactobacillus rhamnosus Gorbach-Goldin (LGG) is known for its beneficial effects on gut health.

Purpose of the Study:

• To investigate the impact of LGG-derived soluble mediators (LSMs) on intestinal barrier function in a rat model of SBS.
• To compare the efficacy of LSMs with viable LGG in ameliorating SBS-induced intestinal damage.

Main Methods:

• Rats underwent 80% small-bowel resection (SBR) to induce SBS.
• Groups were supplemented with water (SBS), viable LGG, or LSMs.
• Evaluated parameters included body weight, ileum histology, permeability, bacterial translocation, inflammatory cytokines, and tight junction proteins.

Main Results:

• SBR led to increased intestinal permeability and reduced tight junction proteins.
• Both LGG and LSMs decreased bacterial translocation and permeability compared to SBS.
• LSMs showed superior results in body weight gain, reduced endotoxin levels, and enhanced tight junction protein expression (claudin-1, claudin-4) compared to viable LGG.

Conclusions:

• Enteral supplementation with LSMs or viable LGG can improve intestinal barrier function in SBS.
• LSMs effectively mimic the beneficial effects of viable LGG.
• LSMs demonstrate enhanced efficacy in reducing inflammation and restoring intestinal barrier integrity in SBS.