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Absolute Analysis of Nanoparticle Suspension with Surface Plasmon Microscopy
Alexey Kuzmichev1, Julija Skolnik1, Alexander Zybin1
1Leibniz-Institut für Analytische Wissenschaften - ISAS - e.V. , Bunsen-Kirchhoff-Straße 11 , 44139 Dortmund , Germany.
Analytical Chemistry
|August 23, 2018
Summary
Surface plasmon microscopy enables absolute nanoparticle analysis by monitoring particle transfer to a sensor. This method offers a powerful tool for nanoparticle analytics and binding process investigations.
Area of Science:
- Nanotechnology
- Analytical Chemistry
- Physical Chemistry
Background:
- Surface plasmon microscopy (SPM) is a recent technique for observing single nanoparticles.
- SPM allows monitoring of particle transfer from solution to a surface.
Purpose of the Study:
- To examine the feasibility of absolute nanoparticle concentration analysis using SPM.
- To develop a model for quantifying nanoparticle concentration based on binding events.
Main Methods:
- A model based on particle diffusion in laminar flow was developed.
- Mass transport equations were solved analytically.
- Particle binding to the sensor surface was observed using SPM.
Main Results:
- Direct determination of particle concentration is possible by counting bound particles.
- The model utilizes independent parameters: flow cell geometry, flow rate, particle size, and viscosity.
- Irreversible binding is achievable within a specific electrolyte concentration range.
Conclusions:
- The study demonstrates the feasibility of absolute nanoparticle analysis using SPM.
- The method shows great potential for comprehensive nanoparticle analytics and binding studies.
- The model can be extended to account for binding probabilities less than 100%.
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