Related Experiment Video

Updated: Feb 6, 2026

Bone Marrow-derived Macrophage Production
07:06

Bone Marrow-derived Macrophage Production

Published on: November 22, 2013

75.3K

Analysis of Necroptosis in Bone Marrow-Derived Macrophages

Diana Legarda1, Adrian T Ting2

  • 1Precision Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA. diana.legarda@mssm.edu.

Methods in Molecular Biology (Clifton, N.J.)
|August 24, 2018
PubMed

Insights

Necroptosis in macrophages drives inflammation in various diseases. This study details methods to isolate quiescent macrophages for studying necroptosis regulation and quantification.

Area of Science:

  • Immunology
  • Cellular Biology
  • Pathology

Background:

  • Necroptosis, a form of programmed cell death, is implicated in inflammatory pathologies.
  • Macrophages play a crucial role in the inflammatory response and are involved in necroptosis.

Purpose of the Study:

  • To present a reliable method for obtaining quiescent, mature macrophages.
  • To establish techniques for quantifying necroptosis in these cells.
  • To facilitate research into the molecular mechanisms regulating necroptosis in macrophages.

Main Methods:

  • Isolation of quiescent, mature macrophages using a well-established protocol.
  • Application of two distinct quantitative assays to measure necroptosis.

Main Results:

  • Successfully obtained quiescent, mature macrophages suitable for necroptosis studies.
  • Validated two methods for quantifying necroptosis in macrophages.

Conclusions:

  • The described methods provide a foundation for investigating necroptosis in macrophages.
  • This work aids in understanding the role of macrophage necroptosis in inflammatory diseases.
Keywords:
ATP viability assayBone marrow-derived macrophagesCell deathLuminescenceNecroptosisPropidium iodide exclusion

More Related Videos

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages10:07

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Published on: June 23, 2013

68.5K
Author Spotlight: Developing a Reproducible Method for Isolating Bone Marrow-Derived Macrophages from Neonatal Mice to Advance Early Life Immune Responses06:53

Author Spotlight: Developing a Reproducible Method for Isolating Bone Marrow-Derived Macrophages from Neonatal Mice to Advance Early Life Immune Responses

Published on: May 24, 2024

3.0K

Related Concept Videos

Bone Marrow Sampling and Transplants01:22

Bone Marrow Sampling and Transplants

Bone marrow transplant is a potential cure for several diseases, including cancer and specific genetic disorders. Notably, this procedure is applicable for patients suffering from aplastic anemia, certain types of leukemia, severe combined immunodeficiency disease (SCID), Hodgkin's disease, non-Hodgkin's lymphoma, multiple myeloma, thalassemia, sickle-cell disease, and certain cancers.
The transplant begins with high doses of chemotherapy and radiation treatment, which aim to destroy...
1.2K
Bone Structure01:55

Bone Structure

Within the skeletal system, the structure of a bone, or osseous tissue, can be exemplified in a long bone, like the femur, where there are two types of osseous tissue: cortical and cancellous.
51.8K
Bone Remodeling01:40

Bone Remodeling

Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
40.5K
Compact Bone01:27

Compact Bone

Most bones contain compact and spongy osseous tissue, but their distribution and concentration vary based on the bone's overall function.
Compact bone, also called cortical bone, is the denser, stronger of the two types of bone tissue. It is found under the periosteum and in the diaphyses of long bones, where it provides support and protection. The microscopic structural unit of compact bone is called an osteon, or haversian system. Each osteon is composed of concentric rings of calcified...
16.6K
Bone Disorders01:29

Bone Disorders

Aging and its effect on bone remodeling is the most common cause of bone disorders. In young and healthy people, bone deposition and resorption happen at an equal rate to maintain optimal bone health.
Bone deposition is also affected by the levels of sex hormones like estrogen and testosterone that promote osteoblast activity and bone matrix synthesis. When the level of these hormones decreases due to aging, it causes a reduction in bone deposition. As a result, bone resorption by osteoclasts...
5.4K
The Hyoid Bone01:12

The Hyoid Bone

The hyoid bone is a small U-shaped bone located in the upper neck at the level of the inferior mandible, with its tips pointing posteriorly. It does not directly articulate with any other bone in the body. The hyoid acts as the attachment site for the tongue, the larynx, and the pharynx. It is held in position by a series of small muscles attached from above or below. These muscles help to move the hyoid up/down or forward/back in coordination with movements of the tongue, larynx, and pharynx...
5.1K