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Updated: Feb 6, 2026

Characterization of MLKL-mediated Plasma Membrane Rupture in Necroptosis
08:55

Characterization of MLKL-mediated Plasma Membrane Rupture in Necroptosis

Published on: August 7, 2018

11.3K

Detection of MLKL Oligomerization During Programmed Necrosis

Zhenyu Cai1,2, Zheng-Gang Liu3

  • 1Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA.

Methods in Molecular Biology (Clifton, N.J.)
|August 24, 2018
PubMed

Insights

This study details a Western blotting method to detect mixed lineage kinase domain-like protein (MLKL) oligomerization, a key step in programmed necrosis (necroptosis). This protocol aids in identifying the activation of the necroptosis pathway.

Area of Science:

  • Cellular Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Programmed necrosis, or necroptosis, is a regulated form of cell death.
  • It involves receptor-interacting protein kinases (RIPK1, RIPK3) and mixed lineage kinase domain-like protein (MLKL).
  • MLKL activation, specifically oligomerization, is crucial for executing necroptosis.

Purpose of the Study:

  • To establish a detailed protocol for detecting MLKL oligomerization.
  • To provide a method for assessing the activation of the programmed necrosis pathway.

Main Methods:

  • Western blotting analysis under nonreducing conditions.
  • Detection of MLKL oligomerization in cells undergoing necroptosis.

Main Results:

  • A reliable method to detect MLKL oligomerization was established.
  • The protocol allows for the assessment of programmed necrosis pathway activation.

Conclusions:

  • The developed Western blotting protocol effectively detects MLKL oligomerization.
  • This method is valuable for studying necroptosis and its regulatory mechanisms.
Keywords:
ImmunoblotsMLKLNecroptosisNonreducing SDS-PAGE

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