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Assessment of Kidney Function in Mouse Models of Glomerular Disease
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Assessment of Kidney Function in Mouse Models of Glomerular Disease

Published on: June 30, 2018

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Assessment of In Vivo Kidney Cell Death: Glomerular Injury

Wulf Tonnus1, Moath Al-Mekhlafi1, Florian Gembardt1

  • 1Division of Nephrology, Department of Internal Medicine III, University Hospital Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|August 24, 2018
PubMed

Insights

Investigating glomerular cell death is crucial for understanding kidney diseases like nephrotic syndrome and FSGS. This study details methods to study cell death in mesangial, endothelial, and podocyte cells in vivo.

Area of Science:

  • Nephrology
  • Cell Biology
  • Pathology

Background:

  • The glomerulus, the kidney's filtration unit, comprises mesangial, endothelial, and podocyte cells.
  • These glomerular cells are implicated in various kidney diseases, including nephrotic syndrome, nephritic syndrome, and focal segmental glomerulosclerosis (FSGS).
  • Cell death mechanisms in these critical cell types remain understudied but are vital for understanding disease pathogenesis.

Purpose of the Study:

  • To investigate the role of cell death in glomerular pathologies.
  • To present protocols for targeting glomerular cells in vivo for research purposes.
  • To elucidate mechanisms of cell death in mesangial, endothelial, and podocyte cells relevant to kidney disease.

Main Methods:

  • Utilizing artificial antisera to induce specific cell injury (e.g., anti-mesangial cell serum-induced mesangiolysis, endothelial cell injury).
  • Examining genetic variations leading to podocyte dysfunction and nephrotic syndrome.
  • Developing and applying in vivo protocols to target and study glomerular cells.

Main Results:

  • Cell death in glomerular cells is a key factor in the pathogenesis of various kidney diseases.
  • Artificial antisera effectively induce specific glomerular cell damage, providing models for study.
  • Genetic podocyte dysfunction models share cell death mechanisms with secondary FSGS.

Conclusions:

  • Understanding glomerular cell death is essential for advancing treatments for kidney diseases.
  • The presented in vivo protocols offer valuable tools for studying glomerular cell biology and pathology.
  • Targeting cell death pathways in glomerular cells holds therapeutic potential for nephrotic syndrome and other glomerular disorders.

Keywords:
Anti-mesangial cell serum-induced mesangiolysisSecondary FSGS modelSelective endothelial cell injury model

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