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LFchimera protects HeLa cells from invasion by Yersinia spp. in vitro

Tjitske Sijbrandij1, Antoon J Ligtenberg1, Kamran Nazmi1

  • 1Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam, University of Amsterdam and VU University Amsterdam, Gustav Mahlerlaan 3004, 1081 LA, Amsterdam, The Netherlands.

Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
|August 24, 2018
PubMed

Insights

The peptide LFchimera shows potential against Yersinia infections by inhibiting bacterial invasion of host cells. This study explored LFchimera

Area of Science:

  • Microbiology
  • Immunology
  • Biochemistry

Background:

  • Yersinia pestis causes plague, necessitating alternative therapies due to limited antibiotics and no vaccines.
  • Antimicrobial peptides offer a promising avenue for novel therapeutic strategies.
  • LFchimera, derived from bovine lactoferrin, exhibits broad-spectrum antimicrobial activity.

Purpose of the Study:

  • To evaluate the therapeutic potential of LFchimera against Yersinia enterocolitica and Yersinia pseudotuberculosis in vitro.
  • To investigate LFchimera's effect on Yersinia spp. adherence and invasion of host cells.
  • To determine the mechanism underlying LFchimera's impact on host-pathogen interactions.

Main Methods:

  • In vitro assays using Yersinia enterocolitica and Yersinia pseudotuberculosis as simulants for Yersinia pestis.
  • Assessment of LFchimera's antimicrobial activity and its effects on bacterial invasion of human HeLa epithelial cells.
  • Analysis of host-cell mediated responses, including pro-inflammatory cytokine release upon exposure to LFchimera and Yersinia spp.

Main Results:

  • LFchimera demonstrated susceptibility against Yersinia enterocolitica and Yersinia pseudotuberculosis in vitro.
  • LFchimera effectively inhibited the invasion of host cells by Yersinia spp. in a host-cell mediated manner.
  • Co-exposure to LFchimera and Yersinia strains induced pro-inflammatory cytokine release from human epithelial cells.

Conclusions:

  • LFchimera exhibits therapeutic potential against Yersinia infections by interfering with host cell invasion.
  • The study highlights a host-cell mediated mechanism for LFchimera's anti-invasion activity.
  • LFchimera's ability to modulate host inflammatory responses warrants further investigation for plague treatment strategies.
Keywords:
Antimicrobial peptideBiowarfare simulantsCellular adhesion and invasionLFchimeraLactoferrin

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