[A cross-sequence labeling equipment for high-throughput proteome based on pseudo-isobaric dimethyl labeling]

Area of Science:

• Proteomics
• Biotechnology
• Analytical Chemistry

Background:

• High-throughput proteome quantification is crucial for biology and precision medicine.
• Existing methods face challenges in multi-sample analysis and operational efficiency.

Purpose of the Study:

• To develop a novel, high-throughput proteome labeling equipment for quantitative analysis.
• To improve the efficiency, accuracy, and operability of proteome labeling.

Main Methods:

• Established a pseudo-isobaric dimethyl labeling (pIDL-StageTip) system utilizing simple devices and centrifugal force.
• Optimized labeling conditions using NaBD3CN and NaBH3CN under acidic conditions.
• Validated the method on standard proteolysis and complex human proteome samples.

Main Results:

• Achieved 100% labeling efficiency and >95% selectivity on standard proteolysis.
• Demonstrated >99% labeling efficiency and 100% selectivity in complex human proteome.
• The developed equipment showed high quantitative accuracy and precision.

Conclusions:

• The pIDL-StageTip equipment provides a reliable solution for high-throughput proteome labeling.
• The system offers enhanced operability, accuracy, and throughput for quantitative proteomics.
• This advancement supports multidimensional and multi-sample proteome quantification needs.