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Updated: Feb 6, 2026

Phase Diagram Characterization Using Magnetic Beads as Liquid Carriers
Published on: September 4, 2015
[Preparation and application of magnetic reversed-phase restricted access material]
Shanshan Wu1, Chanling Wei1, Lijuan Zhao1
1School of Chemistry and Chemical Engineering, North Minzu University, Yinchuan, 750021, China.
Abstract:
Using magnetic silica (Fe2O3@SiO2) as the matrix material, a magnetic reversed-phase restricted access material was prepared via surface-initiation atom transfer radical polymerization (SI-ATRP) on the surface of modified Fe2O3@SiO2. Stearyl methacrylate (SMA) was grafted on the inner surface of the modified Fe2O3@SiO2, and glycidylmethacrylate (GMA) was grafted on the outer surface of the modified Fe2O3@SiO2. The magnetic reversed-phase restricted access material was prepared via acid hydrolysis. This material was characterized by scanning electron microscope (SEM), X-ray diffraction (XRD), transmission electron microscopy (TEM), vibrating sample magnetometer (VSM) and elemental analysis. The exclusion ability of the opened magnetic reversed-phase restricted access material to bovine serum albumin (BSA) was 90.4%. The maximum adsorbed amounts of sulfisoxazole (SIZ), sulfadimethoxine (SDM), trimethoprim (TMP) and sulfamerazine (SMR) were 2.76, 2.24, 1.51 and 1.34 mg/g, respectively. This material was applied to extract and enrich SIZ, SMR and SDM in milk and bovine serum samples. The spiked recoveries of SIZ, SMR and SDM were 88.7%-90.8%, and the relative standard deviations were 3.3%-5.3%. Thus, the magnetic phase restricted access material can simplify the pretreatment of biological matrix samples, and can be applied to the analysis and detection of blood samples or food samples.
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