Analyzing Mechanotransduction Through the LINC Complex in Isolated Nuclei
Nejma Belaadi1, Angélique Millon-Frémillon2, Julien Aureille2
1l'institut du thorax, Inserm, CNRS, Université de Nantes, Nantes, France.
Abstract:
The mechanical properties of the cellular microenvironment can impact many aspects of cell behavior, including molecular processes in the nucleus. Recent studies indicate that the LINC complex and its associated nuclear envelope transmit and transduce mechanical stress into biochemical pathways that ultimately regulate nuclear structure or gene expression. Here we describe a method to apply tensional forces to the LINC complex of isolated nuclei. Using magnetic beads and magnets, this technique can be used to explore the biochemical pathways that are activated in response to tension applied to the surface of isolated nuclei.
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