Related Experiment Video
Updated: Feb 6, 2026
03:55
A Sensitive Visual Method for the Detection of Hydrogen Sulfide Producing Bacteria
Published on: June 27, 2022
4.2K
H2S biotreatment with sulfide-oxidizing heterotrophic bacteria
Ningke Hou1, Yongzhen Xia1, Xia Wang1
1State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, People's Republic of China.
Biodegradation
|August 25, 2018
Summary
Heterotrophic bacteria can efficiently remove toxic hydrogen sulfide (H2S) by oxidizing it to elemental sulfur and thiosulfate. Immobilization with iron oxide nanoparticles further enhances this H2S biotreatment capability.
Area of Science:
- Environmental microbiology
- Biotechnology
- Bioremediation
Background:
- Hydrogen sulfide (H2S) is a toxic and odorous industrial byproduct.
- Chemolithotrophic bacteria are traditionally used for H2S remediation.
- Heterotrophic bacteria possess enzymes like sulfide:quinone oxidoreductase for H2S oxidation.
Purpose of the Study:
- To investigate the potential of heterotrophic bacteria for H2S biotreatment.
- To evaluate the efficiency of cultured heterotrophic bacteria in oxidizing H2S.
- To assess the impact of immobilization and nanoparticles on H2S removal.
Main Methods:
- Culturing heterotrophic bacteria with organic compounds.
- Measuring H2S oxidation rates in bacterial suspensions.
- Testing immobilized bacteria with and without Fe3O4 nanoparticles.
Main Results:
- Heterotrophic bacteria rapidly oxidized H2S to zero-valence sulfur and thiosulfate without acidification.
- Observed sulfide oxidation rates ranged from 8 to 50 µmol min⁻¹ g⁻¹ cell dry weight.
- Immobilized bacteria, especially with Fe3O4 nanoparticles, showed efficient H2S removal.
Conclusions:
- Heterotrophic bacteria are effective for H2S biotreatment under aerobic conditions.
- Immobilization and Fe3O4 nanoparticles enhance H2S removal efficiency.
- This approach offers a promising alternative for industrial H2S remediation.
More Related Videos
Related Concept Videos
02:26Preparation and Reactions of Sulfides
5.8K
Sulfides are the sulfur analog of ethers, just as thiols are the sulfur analog of alcohol. Like ethers, sulfides also consist of two hydrocarbon groups bonded to the central sulfur atom. Depending upon the type of groups present, sulfides can be symmetrical or asymmetrical. Symmetrical sulfides can be prepared via an SN2 reaction between 2 equivalents of an alkyl halide and one equivalent of sodium sulfide.
5.8K
03:14Oxidation Numbers
42.8K
In redox reactions, the transfer of electrons occurs between reacting species. Electron transfer is described by a hypothetical number called the oxidation number (or oxidation state). It represents the effective charge of an atom or element, which is assigned using a set of rules.
42.8K
02:17Structure and Nomenclature of Thiols and Sulfides
5.7K
Thiols and sulfides are sulfur analogs of alcohols and ethers, respectively, where the sulfur atom takes the place of the oxygen atom. Thus, thiols are generally represented as RSH, where R is an alkyl substituent and —SH is the functional group. On the other hand, in sulfides, the central sulfur atom is bonded to two hydrocarbon groups on either side. Depending upon the type of group, sulfides can be either symmetrical or asymmetrical. Both thiols and sulfides display a bent geometry,...
5.7K
01:15Pyruvate Oxidation
169.0K
After glycolysis, the charged pyruvate molecules enter the mitochondria via active transport and undergo three enzymatic reactions. These reactions ensure that pyruvate can enter the next metabolic pathway so that energy stored in the pyruvate molecules can be harnessed by the cells.
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
169.0K
03:11Oxidation-Reduction Reactions
75.7K
Oxidation–Reduction Reactions
75.7K
02:37Oxidation of Alcohols
16.2K
In this lesson, the oxidation of alcohols is discussed in depth. The various reagents used for oxidation of primary and secondary alcohols are detailed, and their mechanism of action is provided.
The process of oxidation in a chemical reaction is observed in any of the three forms:
The process of oxidation in a chemical reaction is observed in any of the three forms:
16.2K