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A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging
Published on: April 8, 2019
Large field of view correction by using conjugate adaptive optics with multiple guide stars
Qi Zhao1,2, Xin Shi2, Xinpei Zhu1
1Center for Neuroscience, Department of Neurobiology, the Second Affiliated Hospital, Zhejiang, University School of Medicine, Hangzhou, Zhejiang, China.
Abstract:
Adaptive optics has been widely used in the optical microscopy to recover high-resolution images deep into the sample. However, the corrected field of view (FOV) with a single correction is generally limited, which seriously restricts the imaging speed. In this article, we demonstrate a high-speed wavefront correction method by using the conjugate adaptive optical correction with multiple guide stars (CAOMG) based on the coherent optical adaptive technique. The results show that the CAOMG method can greatly improve the corrected FOV. For 120-μm-thick mouse brain tissue, the corrected FOV can be improved up to ~243 times of the conventional pupil adaptive optics (PAO) without additional time consumption. Therefore, this study shows the potential of high-speed imaging through scattering medium in biological science.
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