Electrochemical inactivation of bacteria with a titanium sub-oxide reactive membrane

A reactive electrochemical membrane (REM) system was developed with titanium suboxide microfiltration membrane serving as the filter and the anode, and was examined to inactivate Escherichia coli (E. coli) in water at various current densities. After passing through the membrane filter, the concentration of E. coli decreased from 6.46 log CFU/mL to 0.18 log CFU/mL. The REM operation and effects, including membrane pressure, anode potential, protein leakage, and cell morphology, were characterized under different treatment conditions. It was found that several mechanisms, including membrane filtration, external electrical field influence, and direct oxidation, functioned in concert to lead to bacteria removal and inactivation, and direct oxidation likely played the major role. As revealed by scanning electron microscope and extracellular protein analysis, high current density and voltage caused severe cell damage that resulted in partial or complete cell disintegration. The removal of a model virus, bacteriophage MS2, was also investigated at the current density of 10 mA cm^-2 and achieved 6.74 log reduction compared to the original concentration (10¹¹ PFU/mL). In addition to illustration of mechanisms, this study may provide a potentially promising approach that is suitable for decentralized treatment to meet dispersed water disinfection needs.