Direct Generation of Human Cortical Organoids from Primary Cells
Related Experiment Video
Updated: Feb 6, 2026

Generation of Multicellular Human Primary Endometrial Organoids
Published on: October 4, 2019
Monique Schukking1,2, Helen C Miranda1,2, Cleber A Trujillo1,2
11 Department of Pediatrics, Rady Children's Hospital San Diego, University of California San Diego School of Medicine , La Jolla, California.
Abstract:
The study of variations in human neurodevelopment and cognition is limited by the availability of experimental models. While animal models only partially recapitulate the human brain development, genetics, and heterogeneity, human-induced pluripotent stem cells can provide an attractive experimental alternative. However, cellular reprogramming and further differentiation techniques are costly and time-consuming and therefore, studies using this approach are often limited to a small number of samples. In this study, we describe a rapid and cost-effective method to reprogram somatic cells and the direct generation of cortical organoids in a 96-well format. Our data are a proof-of-principle that a large cohort of samples can be generated for experimental assessment of the human neural development.
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