Biocompatible silk/calcium silicate/sodium alginate composite scaffolds for bone tissue engineering
Ao Zheng1, Lingyan Cao1, Yang Liu2
1Department of Prosthodontics, Oral Bioengineering and Regenerative Medicine Lab, Shanghai Key Laboratory of Stomatology, Ninth People's Hospital Affiliated to Shanghai JiaoTong University, School of Medicine, 639 Zhizaoju Road, Shanghai 200011, China; Oral Bioengineering Lab, Shanghai Research Institute of Stomatology, Ninth People's Hospital Affiliated to Shanghai JiaoTong University, School of Medicine, Key Laboratory of Stomatology, Shanghai 200011, China.
Carbohydrate Polymers
|August 26, 2018
Summary
This study presents novel silk fibroin/calcium silicate/sodium alginate scaffolds for bone tissue engineering. These scaffolds enhance cell proliferation and activity, showing promise for non-loading applications.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Scaffolds are critical in bone tissue engineering, influencing seeded cell behavior.
- Existing scaffolds often require optimization for enhanced biological and mechanical properties.
Purpose of the Study:
- To develop and evaluate novel interpenetrating network hydrogel scaffolds for bone tissue engineering.
- To investigate the effect of calcium silicate incorporation on scaffold properties and cellular response.
Main Methods:
- Fabrication of silk fibroin (SF), sodium alginate (SA), and calcium silicate (CS) interpenetrating network hydrogel scaffolds.
- Lyophilization to form porous scaffolds, followed by physical characterization.
- In vitro assessment of cytocompatibility and alkaline phosphatase (ALP) activity using bone marrow stromal cells (BMSCs).
Main Results:
- Calcium silicate incorporation significantly enhanced scaffold hydrophilicity, degradation, compression resistance, bioactivity, and pH.
- SF/CS/SA scaffolds, particularly those with 25% and 12% CS, markedly stimulated BMSC proliferation.
- BMSCs cultured on 25/CS and 12/CS scaffolds exhibited significantly high ALP activity, indicating osteogenic differentiation.
Conclusions:
- SF/CS/SA scaffolds demonstrate excellent physical and biological properties for bone tissue engineering.
- The developed scaffolds show significant potential for non-loading bone regeneration applications.
- Calcium silicate is a valuable component for enhancing the performance of SF/SA based bone tissue engineering scaffolds.
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