Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Emission Spectra02:39

Emission Spectra

76.4K
When solids, liquids, or condensed gases are heated sufficiently, they radiate some of the excess energy as light. Photons produced in this manner have a range of energies, and thereby produce a continuous spectrum in which an unbroken series of wavelengths is present.
76.4K
Imaging Studies II: Positron Emission Tomography and Scintigraphy01:25

Imaging Studies II: Positron Emission Tomography and Scintigraphy

597
Positron Emission Tomography (PET) is a medical imaging technique that provides crucial insights into the body's physiological functions at a molecular level. It is an indispensable resource for diagnosing, staging, and monitoring various illnesses, notably cancer, neurological disorders, and cardiovascular conditions.
Fundamental Principles of PET
597
The Extracellular Matrix01:42

The Extracellular Matrix

89.1K
Overview
89.1K
The Extracellular Matrix01:29

The Extracellular Matrix

12.3K
Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
12.3K
Positron Emission Tomography01:29

Positron Emission Tomography

7.5K
Positron emission tomography (PET) is a medical imaging technique involving radiopharmaceuticals — substances that emit short-lived radiation. Although the first PET scanner was introduced in 1961, it took 15 more years before radiopharmaceuticals were combined with the technique and revolutionized its potential.
One of the main requirements of a PET scan is a positron-emitting radioisotope, which is produced in a cyclotron and then attached to a substance used by the part of the body...
7.5K
Excitation-Contraction Coupling in Skeletal Muscles01:20

Excitation-Contraction Coupling in Skeletal Muscles

15.2K
Excitation-contraction coupling is a series of events that occur between generating an action potential and initiating a muscle contraction. It occurs at the triad, a structure found in skeletal muscle fibers that comprise a T-tubule and terminal cisternae of the sarcoplasmic reticulum on each side. These triads are visible in longitudinally sectioned muscle fibers. They are typically located at the A-I junction — the junction between the A and I bands of the sarcomere.
When an action...
15.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chemometric electrochemical fingerprinting of thermal stress in honeybee larvae: a tool for discrimination and welfare assessment.

Analytical and bioanalytical chemistry·2026
Same author

Ciprofloxacin and glyphosate co-exposure alters soybean development and reprograms metabolic pathways.

Environmental science and pollution research international·2026
Same author

Multi-way data modelling for enhancing classification performance: Fluorescence data as a case of study.

Analytica chimica acta·2026
Same author

Comprehensive chemical fingerprinting by LC×LC-fluorescence and data-driven chemometric modelling for unsupervised classification.

Talanta·2025
Same author

Functional data analysis, a comprehensive framework for processing non-quadrilinear and low-selective data provided by four-way liquid chromatography analysis.

Analytica chimica acta·2025
Same author

Unambiguous Determination of Benzo[a]pyrene and Dibenzo[a,l]pyrene in HPLC Fractions via Room-Temperature Fluorescence Excitation-Emission Matrices.

Molecules (Basel, Switzerland)·2025

Related Experiment Video

Updated: Feb 6, 2026

Author Spotlight: Functional Site-Directed Fluorometry in Native Cells to Study Skeletal Muscle Excitability
12:26

Author Spotlight: Functional Site-Directed Fluorometry in Native Cells to Study Skeletal Muscle Excitability

Published on: June 2, 2023

1.5K

Multiway analysis through direct excitation-emission matrix imaging.

Mirta R Alcaraz1, Ezequiel Morzán2, Cecilia Sorbello3

  • 1Departamento de Química Inorgánica, Analítica y Química Física, INQUIMAE, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes 2160, Ciudad Universitaria, Pabellón 2, Buenos Aires, C1428EGA, Argentina; Laboratorio de Desarrollo Analítico y Quimiometría (LADAQ), Cátedra de Química Analítica I, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Ciudad Universitaria, Santa Fe, S3000ZAA, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz, 2290, Buenos Aires, C1425FQB, Argentina.

Analytica Chimica Acta
|August 26, 2018
PubMed
Summary

This study introduces a rapid in-flow system for fluorescence excitation-emission matrices, enabling measurements in milliseconds. The developed method accurately analyzes complex mixtures and demonstrates potential for quantitative analysis and chromatographic monitoring.

Keywords:
ChemometricsExcitation-emission matrixHigher-order data analysisImaging

More Related Videos

Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging
07:41

Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging

Published on: July 19, 2016

8.1K
Assessing Corticospinal Excitability During Goal-Directed Reaching Behavior
05:05

Assessing Corticospinal Excitability During Goal-Directed Reaching Behavior

Published on: December 2, 2022

2.1K

Related Experiment Videos

Last Updated: Feb 6, 2026

Author Spotlight: Functional Site-Directed Fluorometry in Native Cells to Study Skeletal Muscle Excitability
12:26

Author Spotlight: Functional Site-Directed Fluorometry in Native Cells to Study Skeletal Muscle Excitability

Published on: June 2, 2023

1.5K
Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging
07:41

Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging

Published on: July 19, 2016

8.1K
Assessing Corticospinal Excitability During Goal-Directed Reaching Behavior
05:05

Assessing Corticospinal Excitability During Goal-Directed Reaching Behavior

Published on: December 2, 2022

2.1K

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Fluorescence Spectroscopy

Background:

  • Accurate and rapid acquisition of fluorescence data is crucial for analyzing complex mixtures.
  • Traditional methods can be time-consuming, limiting real-time applications.
  • Developing high-throughput fluorescence measurement techniques is an ongoing challenge.

Purpose of the Study:

  • To present a direct in-flow methodology for acquiring excitation-emission fluorescence matrices (EEMs) with high temporal resolution.
  • To demonstrate the system's capability for spectral calibration, data analysis using PARAFAC, and quantitative measurements.
  • To validate the fast data acquisition for chromatographic applications, generating third-order data.

Main Methods:

  • A direct in-flow system utilizing a continuous light source, gratings, and a CCD array sensor for EEM acquisition.
  • Spatially resolved excitation and emission collection within a square-section capillary.
  • Spectral calibration using interference filters (340-740 nm) and analysis of second-order data.
  • Application of PARAFAC (Parallel Factor Analysis) for trilinearity evaluation and comparison with experimental spectra.
  • Monitoring of chromatographic dye mixtures for third-order LC-EEM data generation.

Main Results:

  • The system achieves measurement times in the tens of milliseconds.
  • Spectral calibration ensures wavelength accuracy.
  • PARAFAC analysis confirms the trilinearity of the data and agreement between experimental and retrieved spectra.
  • Demonstrated feasibility for quantitative analysis using second-order data.
  • Successful generation of third-order LC-EEM data, showing improved resolution in chromatographic analysis.

Conclusions:

  • The developed in-flow methodology provides a rapid and accurate approach for EEM acquisition.
  • The system is suitable for analyzing complex mixtures and quantitative applications.
  • The ability to generate third-order data enhances chromatographic analysis resolution.