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Lactate transport facilitates neurite outgrowth
Kun Chen1,2, Peng Cheng1,2, Huan Wang1,2
1Biological Engineering and Regenerative Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Bioscience Reports
|August 26, 2018
Summary
Lactate transport is crucial for neural development. Disrupting lactate production or transport in glial cells and neurons impairs neurite outgrowth, but L-lactate can restore growth.
Area of Science:
- Neuroscience
- Cell Biology
- Developmental Biology
Background:
- Glia's role in neurite outgrowth is not fully understood.
- Lactate metabolism is essential for neuronal function.
Purpose of the Study:
- To investigate the role of lactate production and transport in neurite outgrowth.
- To elucidate the molecular mechanisms by which glia influence neuronal development.
Main Methods:
- Inhibition of lactate production using 1,4-dideoxy-1,4-imino-D-arabinitol (DAB) and isofagomine.
- Exogenous application of L-lactate.
- Monocarboxylate transporter-2 knockout.
- Analysis of Akt and glycogen synthase kinase 3β (GSK3β) activity.
- GSK3βSer9 mutation.
Main Results:
- Disrupting lactate production significantly interfered with neurite outgrowth.
- Exogenous L-lactate rescued neurite growth.
- Monocarboxylate transporter-2 knockout decreased axon and dendrite length.
- Astrocytic glycogen phosphorylase blockade decreased Akt and increased GSK3β activity.
- GSK3βSer9 mutation reversed growth failure.
Conclusions:
- Lactate transportation is critical for neural development.
- Disruption of the lactate shuttle affects neurite outgrowth in the central nervous system.
Keywords:
AktGlycogen synthase kinase 3β (GSK-3β)L-lactateMonocarboxylate transporters-2 (MCT-2)Neurite outgrowthMore Related Videos
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